Diani A R, Sawada G A, Peterson T, Wyse B M, Blanks M C, Vidmar T J, Gerritsen G C
Microvasc Res. 1986 May;31(3):306-16. doi: 10.1016/0026-2862(86)90019-1.
Renal glomeruli of 11- to 15- and 19- to 23-month-old nondiabetic (M) and diabetic (XA and AC) genetic sublines of Chinese hamsters were morphometrically analyzed to determine if minimal capillary basement membrane thickening (CBMT) is a microvascular complication in this animal model. Minimal glomerular capillary basement membrane thickness was significantly elevated in the AC diabetic subline (117.2 nm +/- 5.0, P less than 0.004) compared with the M nondiabetic subline (99.0 nm +/- 14.0) in the 11- to 15-month age span. However, in the 19- to 23-month age range, both the XA (140.2 nm +/- 20.0, P less than 0.02) and AC (140.1 nm +/- 12.4, P less than 0.04) diabetic sublines displayed significantly greater glomerular capillary basement membrane thickness in comparison with the M nondiabetic subline (119.0 nm +/- 13.4). The greatest influence on CBMT in the diabetics was shown to be a combination of the aging process and severity of hyperglycemia. This initial systematic morphometric study has demonstrated that glomerular CBMT is a characteristic microvascular lesion in 11- to 15-month-old diabetic AC and 19- to 23-month-old diabetic XA and AC Chinese hamsters in comparison with age-matched nondiabetics. Furthermore, this investigation suggests that the Chinese hamster appears to be an acceptable model for the study of chronic complications associated with diabetic nephropathy.
对11至15月龄以及19至23月龄的中国仓鼠非糖尿病（M）和糖尿病（XA和AC）遗传亚系的肾小球进行形态计量分析，以确定最小毛细血管基底膜增厚（CBMT）是否为该动物模型中的一种微血管并发症。在11至15月龄期间，与M非糖尿病亚系（99.0 nm±14.0）相比，AC糖尿病亚系的最小肾小球毛细血管基底膜厚度显著增加（117.2 nm±5.0，P<0.004）。然而，在19至23月龄范围内，与M非糖尿病亚系（119.0 nm±13.4）相比，XA（140.2 nm±20.0，P<0.02）和AC（140.1 nm±12.4，P<0.04）糖尿病亚系均显示出显著更大的肾小球毛细血管基底膜厚度。结果表明，对糖尿病患者CBMT影响最大的是衰老过程和高血糖严重程度的综合作用。这项初步的系统形态计量学研究表明，与年龄匹配的非糖尿病仓鼠相比，肾小球CBMT是11至15月龄糖尿病AC以及19至23月龄糖尿病XA和AC中国仓鼠的一种特征性微血管病变。此外，这项研究表明中国仓鼠似乎是研究糖尿病肾病相关慢性并发症的一个可接受的模型。
