Hung Po-Hsiang, Liao Chia-Wei, Ko Fu-Hsuan, Tsai Huai-Kuang, Leu Jun-Yi
Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei 115, Taiwan.
Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan.
iScience. 2023 Apr 10;26(5):106635. doi: 10.1016/j.isci.2023.106635. eCollection 2023 May 19.
Enhanced phenotypic diversity increases a population's likelihood of surviving catastrophic conditions. Hsp90, an essential molecular chaperone and a central network hub in eukaryotes, has been observed to suppress or enhance the effects of genetic variation on phenotypic diversity in response to environmental cues. Because many Hsp90-interacting genes are involved in signaling transduction pathways and transcriptional regulation, we tested how common Hsp90-dependent differential gene expression is in natural populations. Many genes exhibited Hsp90-dependent strain-specific differential expression in five diverse yeast strains. We further identified transcription factors (TFs) potentially contributing to variable expression. We found that on Hsp90 inhibition or environmental stress, activities or abundances of Hsp90-dependent TFs varied among strains, resulting in differential strain-specific expression of their target genes, which consequently led to phenotypic diversity. We provide evidence that individual strains can readily display specific Hsp90-dependent gene expression, suggesting that the evolutionary impacts of Hsp90 are widespread in nature.
增强的表型多样性增加了种群在灾难性条件下存活的可能性。热休克蛋白90（Hsp90）是一种必需的分子伴侣，也是真核生物中的一个核心网络枢纽，已观察到它能响应环境线索抑制或增强遗传变异对表型多样性的影响。由于许多与Hsp90相互作用的基因参与信号转导途径和转录调控，我们测试了Hsp90依赖的差异基因表达在自然种群中的普遍程度。在五种不同的酵母菌株中，许多基因表现出Hsp90依赖的菌株特异性差异表达。我们进一步鉴定了可能导致可变表达的转录因子（TFs）。我们发现，在抑制Hsp90或环境胁迫下，依赖Hsp90的转录因子的活性或丰度在不同菌株间存在差异，导致其靶基因的菌株特异性差异表达，进而导致表型多样性。我们提供的证据表明，单个菌株能够轻易地表现出特定的Hsp90依赖的基因表达，这表明Hsp90在自然界中的进化影响广泛存在。
