Meng Lingwei, Huang Chuangxin, Liu Xin, Qu Hongyi, Wang Qiuliang
School of Rare Earth, University of Science and Technology of China, Hefei, China.
Ganjiang Innovation Academy, Chinese Academy of Science, Ganzhou, China.
Front Bioeng Biotechnol. 2023 Apr 17;11:1167340. doi: 10.3389/fbioe.2023.1167340. eCollection 2023.
Titanium (Ti) and Ti-based alloy materials are commonly used to develop artificial hearts. To prevent bacterial infections and thrombus in patients with implanted artificial hearts, long-term prophylactic antibiotics and anti-thrombotic drugs are required, and this may lead to health complications. Therefore, the development of optimized antibacterial and antifouling surfaces for Ti-based substrate is especially critical when designing artificial heart implants. In this study, polydopamine and poly-(sulfobetaine methacrylate) polymers were co-deposited to form a coating on the surface of Ti substrate, a process initiated by Cu metal ions. The mechanism for the fabrication of the coating was investigated by coating thickness measurements as well as Ultraviolet-visible and X-ray Photoelectron (XPS) spectroscopy. Characterization of the coating was observed by optical imaging, scanning electron microscope (SEM), XPS, atomic force microscope (AFM), water contact angle and film thickness. In addition, antibacterial property of the coating was tested using () and () as model strains, while the material biocompatibility was assessed by the antiplatelet adhesion test using platelet-rich plasma and cytotoxicity tests using human umbilical vein endothelial cells and red blood cells. Optical imaging, SEM, XPS, AFM, water contact angle, and film thickness tests demonstrated that the coating was successfully deposited on the Ti substrate surface. The biocompatibility and antibacterial assays showed that the developed surface holds great potential for improving the antibacterial and antiplatelet adhesion properties of Ti-based heart implants.
钛（Ti）及钛基合金材料常用于研发人工心脏。为防止植入人工心脏的患者发生细菌感染和血栓形成，需要长期预防性使用抗生素和抗血栓药物，而这可能会导致健康并发症。因此，在设计人工心脏植入物时，开发用于钛基基材的优化抗菌和防污表面尤为关键。在本研究中，聚多巴胺和聚（甲基丙烯酸磺酸甜菜碱）聚合物通过铜金属离子引发的过程共沉积在钛基材表面形成涂层。通过涂层厚度测量以及紫外可见光谱和X射线光电子能谱（XPS）对涂层制备机制进行了研究。通过光学成像、扫描电子显微镜（SEM）、XPS、原子力显微镜（AFM）、水接触角和膜厚对涂层进行表征。此外，以（）和（）为模型菌株测试了涂层的抗菌性能，同时通过使用富血小板血浆的抗血小板粘附试验以及使用人脐静脉内皮细胞和红细胞的细胞毒性试验评估了材料的生物相容性。光学成像、SEM、XPS、AFM、水接触角和膜厚测试表明涂层成功沉积在钛基材表面。生物相容性和抗菌分析表明，所开发的表面在改善钛基心脏植入物的抗菌和抗血小板粘附性能方面具有巨大潜力。
