等离子体增强的单个细胞外囊泡表征。
Plasmon-Enhanced Characterization of Single Extracellular Vesicles.
机构信息
Center for Systems Biology, Massachusetts General Hospital, Boston, MA, USA.
出版信息
Methods Mol Biol. 2023;2668:3-13. doi: 10.1007/978-1-0716-3203-1_1.
Extracellular vesicles (EVs) represent heterogeneous populations of membrane-bound vesicles shed from almost all kinds of cells. Although superior to conventional methods, most newly developed EV sensing platforms still require a certain number of EVs, measuring bulk signals from a group of vesicles. A new analytical approach that enables single EV analysis could be extremely valuable for understanding EVs' subtypes, heterogeneity, and production dynamics during disease development and progression. Here, we describe a new nanoplasmonic sensing platform for sensitive single EV analysis. Termed nPLEX-FL (nano-plasmonic EV analysis with enhanced fluorescence detection), the system amplifies EVs' fluorescence signals using periodic gold nanohole structures, enabling sensitive, multiplexed analysis of single EVs.
细胞外囊泡（EVs）是几乎所有细胞分泌的具有异质性的膜结合囊泡群体。尽管比传统方法优越，但大多数新开发的 EV 传感平台仍然需要一定数量的 EV，从一组囊泡中测量总体信号。一种能够进行单个 EV 分析的新分析方法对于理解 EV 在疾病发展和进展过程中的亚型、异质性和产生动力学可能非常有价值。在这里，我们描述了一种用于敏感的单个 EV 分析的新型纳米等离子体传感平台。该系统称为 nPLEX-FL（使用增强荧光检测的纳米等离子体 EV 分析），它使用周期性的金纳米孔结构放大 EV 的荧光信号，实现了单个 EV 的敏感、多重分析。
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