Center for Systems Biology, Massachusetts General Hospital , 185 Cambridge St, CPZN 5206, Boston, Massachusetts 02114, United States.
Department of Neurology, Massachusetts General Hospital , Boston, Massachusetts 02114, United States.
ACS Nano. 2018 Jan 23;12(1):494-503. doi: 10.1021/acsnano.7b07060. Epub 2018 Jan 4.
Extracellular vesicles (EV) are a family of cell-originating, membrane-enveloped nanoparticles with diverse biological function, diagnostic potential, and therapeutic applications. While EV can be abundant in circulation, their small size (∼4 order of magnitude smaller than cells) has necessitated bulk analyses, making many more nuanced biological explorations, cell of origin questions, or heterogeneity investigations impossible. Here we describe a single EV analysis (SEA) technique which is simple, sensitive, multiplexable, and practical. We profiled glioblastoma EV and discovered surprising variations in putative pan-EV as well as tumor cell markers on EV. These analyses shed light on the heterogeneous biomarker profiles of EV. The SEA technology has the potential to address fundamental questions in vesicle biology and clinical applications.
细胞外囊泡（EV）是一类具有多种生物学功能、诊断潜力和治疗应用的细胞起源的膜包裹纳米颗粒。虽然 EV 在循环中可能很丰富，但其小尺寸（比细胞小约 4 个数量级）需要进行批量分析，从而使许多更细微的生物学探索、起源细胞问题或异质性研究变得不可能。在这里，我们描述了一种简单、灵敏、可多重检测和实用的单细胞 EV 分析（SEA）技术。我们对胶质母细胞瘤 EV 进行了分析，发现 EV 上的假定泛 EV 以及肿瘤细胞标志物存在惊人的差异。这些分析揭示了 EV 异质性生物标志物谱。SEA 技术有可能解决囊泡生物学和临床应用中的基本问题。
