通过表面等离子体共振对细胞外囊泡进行表征

Characterization of Extracellular Vesicles by Surface Plasmon Resonance.

作者信息

Im Hyungsoon, Yang Katherine, Lee Hakho, Castro Cesar M

机构信息

Cancer Program, MGH Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Division of Hematology-Oncology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Boston, MA, 02114, USA.

出版信息

Methods Mol Biol. 2017;1660:133-141. doi: 10.1007/978-1-4939-7253-1_11.

DOI:10.1007/978-1-4939-7253-1_11

PMID:28828653

Abstract

Surface plasmon resonance (SPR ) enables real-time, label-free detection of ligand binding to target receptors immobilized on a sensing surface. SPR has emerged as a promising technique for extracellular vesicle (EV) characterization with its label-free detection scheme, and exquisite sensitivity. Among the various system configurations, nanohole-based SPR sensors are of particular interest because of their simple optical setup, tunability, and scalability. Here, we describe the characterization of circulating EVs or exosomes from human clinical samples using a nanohole-based SPR sensor, named nPLEX (nano-plasmonic exosome) sensing.

摘要

表面等离子体共振（SPR）能够实时、无标记地检测固定在传感表面的配体与靶受体的结合。SPR凭借其无标记检测方案和极高的灵敏度，已成为一种用于细胞外囊泡（EV）表征的有前景的技术。在各种系统配置中，基于纳米孔的SPR传感器因其简单的光学设置、可调性和可扩展性而备受关注。在此，我们描述了使用一种名为nPLEX（纳米等离子体外泌体）传感的基于纳米孔的SPR传感器对来自人类临床样本的循环EV或外泌体进行表征。