Laboratory of Neurophysiology, ULB Neuroscience Institute (UNI), Université Libre de Bruxelles (ULB), Bruxelles, Belgium.
Methods Mol Biol. 2023;2668:277-299. doi: 10.1007/978-1-0716-3203-1_19.
Caenorhabditis elegans is a microscopic model nematode characterized by body transparency and ease of genetic manipulation. Release of extracellular vesicles (EVs) is observed from different tissues; of particular interest are the EVs released by the cilia of sensory neurons. C. elegans ciliated sensory neurons produce EVs that are environmentally released and/or captured by neighboring glial cells. In this chapter, we describe a methodological approach to image the biogenesis, release, and capture of EVs by glial cells in anesthetized animals. This method will allow the experimenter to visualize and quantify the release of ciliary-derived EVs.
秀丽隐杆线虫是一种微小的模式线虫，其特点是身体透明，遗传操作简单。从不同组织中观察到细胞外囊泡（EVs）的释放；特别引人关注的是感觉神经元纤毛释放的 EVs。秀丽隐杆线虫有纤毛的感觉神经元产生的 EVs 是环境释放的，或者被邻近的神经胶质细胞捕获的。在这一章中，我们描述了一种方法学方法，用于在麻醉动物中成像神经胶质细胞中 EVs 的生物发生、释放和捕获。这种方法将使实验者能够可视化和量化纤毛衍生 EVs 的释放。
