Peng Tao, Yuan Yingzhe, Huang Aijun, He Jun, Fu Shimin, Duan Shuo, Yi Long, Yuan Chenyang, Yuan Huizhu, Wang Xuefeng, Zhou Changyong
National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, China.
National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou, China.
Front Microbiol. 2023 Apr 18;14:1119619. doi: 10.3389/fmicb.2023.1119619. eCollection 2023.
Huanglongbing (HLB) is a global devastating citrus disease that is mainly caused by " Liberibacter asiaticus" (Las). It is mostly transmitted by the insect Asian citrus psyllid (ACP, ) in a persistent and proliferative manner. Las traverses multiple barriers to complete an infection cycle and is likely involved in multiple interactions with . However, the protein-protein interactions between Las and are largely unknown. Here, we report on a vitellogenin-like protein (Vg_VWD) in that interacts with a Las flagellum (flaA) protein. We found that was upregulated in Las-infected Silencing of in RNAi silencing significantly increased the Las titer, suggesting that plays an important role in the Las interaction. -mediated transient expression assays indicated that Vg_VWD inhibits BAX- and INF1-triggered necrosis and suppresses the callose deposition induced by flaA in . These findings provide new insights into the molecular interaction between Las and .
黄龙病（HLB）是一种全球性的毁灭性柑橘病害，主要由“亚洲韧皮杆菌”（Las）引起。它大多由亚洲柑橘木虱（ACP）以持久性和增殖性方式传播。Las穿越多个屏障以完成感染周期，并且可能参与与[此处原文缺失相关内容]的多种相互作用。然而，Las与[此处原文缺失相关内容]之间的蛋白质-蛋白质相互作用在很大程度上尚不清楚。在此，我们报道了[此处原文缺失相关内容]中一种与Las鞭毛（flaA）蛋白相互作用的类卵黄原蛋白（Vg_VWD）。我们发现[此处原文缺失相关内容]在受Las感染的[此处原文缺失相关内容]中上调。在[此处原文缺失相关内容]中通过RNA干扰沉默[此处原文缺失相关内容]显著增加了Las滴度，表明[此处原文缺失相关内容]在与Las的相互作用中起重要作用。[此处原文缺失相关内容]介导的瞬时表达分析表明，Vg_VWD抑制BAX和INF1触发的坏死，并抑制flaA在[此处原文缺失相关内容]中诱导的胼胝质沉积。这些发现为Las与[此处原文缺失相关内容]之间的分子相互作用提供了新的见解。
