Schnell K F, Michelson G, Albers C
Pflugers Arch. 1986 Jun;406(6):568-73. doi: 10.1007/BF00584022.
The DMO (5,5-dimethyloxazolidine-2,4-dione) transport was studied in human red blood cells by measuring the 14C-DMO back exchange under self exchange conditions from 14C-DMO labeled cells at 0 degrees C. the unidirectional DMO-flux was linearly related to the DMO concentration up to approx. 100 mM. The unidirectional DMO-flux increases as pH is reduced. The DMO transport is not inhibited by anion transport inhibitors like DIDS, SITS, dipyridamole, phlorhizin, salicylate or butanol. A close correlation between the unidirectional DMO-flux and DMOH, the unionized form of DMO, has been observed suggesting that DMO is transported predominantly by nonionic diffusion. the permeability of DMOH is 9.5 X 10(-6) cm/s (0 degrees C) while the permeability of DMO- cannot be assessed from our data.
通过在0℃下测量14C-DMO标记细胞在自交换条件下的14C-DMO反向交换，研究了人红细胞中5,5-二甲基恶唑烷-2,4-二酮（DMO）的转运。单向DMO通量与DMO浓度呈线性关系，直至约100 mM。随着pH值降低，单向DMO通量增加。DMO转运不受DIDS、SITS、双嘧达莫、根皮苷、水杨酸盐或丁醇等阴离子转运抑制剂的抑制。已观察到单向DMO通量与DMO的非离子化形式DMOH之间存在密切相关性，这表明DMO主要通过非离子扩散进行转运。DMOH的渗透率为9.5×10^(-6) cm/s（0℃），而DMO-的渗透率无法从我们的数据中评估。
