Frayne E G, Kellems R E
Nucleic Acids Res. 1986 May 27;14(10):4113-25. doi: 10.1093/nar/14.10.4113.
Structural features of the transcription termination region for the mouse dihydrofolate reductase gene have been determined and compared with those of several other known termination regions for protein coding genes. A common feature identified among these termination regions was the presence of a 20 bp consensus DNA sequence element (ATCAGAATATAGGAAAGTAGCAAT). The results imply that the 20 bp consensus DNA sequence element is important for signaling RNA polymerase II transcription termination at least in the several vertebrate species investigated. Furthermore, the results suggest that for the dhfr gene and possibly for other genes in mice as well, the potential termination consensus sequence can exist as part of a long interspersed repetitive DNA element.
已确定小鼠二氢叶酸还原酶基因转录终止区域的结构特征，并与其他几个已知的蛋白质编码基因终止区域的结构特征进行了比较。在这些终止区域中发现的一个共同特征是存在一个20bp的共有DNA序列元件（ATCAGAATATAGGAAAGTAGCAAT）。结果表明，至少在所研究的几种脊椎动物物种中，这个20bp的共有DNA序列元件对于信号RNA聚合酶II转录终止很重要。此外，结果表明，对于小鼠的二氢叶酸还原酶基因以及可能的其他基因，潜在的终止共有序列可能作为长散在重复DNA元件的一部分存在。
