紫外线诱导的寡核苷酸和多核苷酸中的8,8-腺嘌呤脱氢二聚体。
Ultraviolet-induced 8,8-adenine dehydrodimers in oligo- and polynucleotides.
作者信息
Gasparro F P, Fresco J R
出版信息
Nucleic Acids Res. 1986 May 27;14(10):4239-51. doi: 10.1093/nar/14.10.4239.
Abstract
Characteristic fluorescence excitation and emission is induced by either acetone-sensitized 313 nm irradiation of mixtures of 8-bromoadenosine and adenosine or 254 nm irradiation of oligo- and polynucleotides containing adenine neighbors. The acetone-sensitized reaction involves cleavage of bromine from 8-bromoadenosine with activation of C-8, leading to formation of an 8,8-adenosine dehydrodimer. Comparable fluorescence properties arise in the unsensitized photoreaction of dApdA, pdApdA, ApA, poly(dA), poly(A), poly(dA.dT), and poly(dA.U). The previously unidentified adenine ultraviolet photoproduct described by Porschke has been isolated as several variants from solutions of pdApdA and poly(dA) irradiated at 254 nm. Based upon fluorescence spectra and mass spectra, these variants are shown to contain the 8,8-adenine dehydrodimer moiety.
摘要
通过丙酮敏化的313nm照射8-溴腺苷和腺苷的混合物，或通过254nm照射含有腺嘌呤相邻碱基的寡核苷酸和多核苷酸，可诱导出特征性的荧光激发和发射。丙酮敏化反应涉及8-溴腺苷中溴的裂解以及C-8的活化，导致形成8,8-腺苷脱氢二聚体。在dApdA、pdApdA、ApA、聚（dA）、聚（A）、聚（dA.dT）和聚（dA.U）的非敏化光反应中也会出现类似的荧光特性。Porschke描述的先前未鉴定的腺嘌呤紫外光产物已从254nm照射的pdApdA和聚（dA）溶液中分离出几种变体。基于荧光光谱和质谱，这些变体显示含有8,8-腺嘌呤脱氢二聚体部分。
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