聚脱氧腺苷酸中的一种特定光反应。
A specific photoreaction in polydeoxyadenylic acid.
作者信息
Pörschke D
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2683-6. doi: 10.1073/pnas.70.9.2683.
Abstract
Ultraviolet photosensitivity of the single-stranded forms of poly(A) and poly(dA) is compared. In contrast to poly(A), poly(dA) is rather sensitive to ultraviolet irradiation. Relatively low doses of ultraviolet light lead to characteristic changes of the ultraviolet and circular dichroism spectra of poly(dA), suggesting formation of a specific photoproduct in poly(dA) that cannot be formed in poly(A). The quantum yields in poly(dA) (varphi = 2.5 x 10(-3)) are about a factor of 10 higher than in poly(A), indicating a high structure specificity. The base-pairing capacity of irradiated probes of poly(dA) to poly(dT) is inhibited. These results demonstrate a relatively high probability of DNA photolesions, that cannot be repaired by simple repair enzyme systems.
摘要
比较了聚腺苷酸（poly(A)）和聚脱氧腺苷酸（poly(dA)）单链形式的紫外线光敏性。与聚腺苷酸不同，聚脱氧腺苷酸对紫外线照射相当敏感。相对低剂量的紫外线会导致聚脱氧腺苷酸的紫外光谱和圆二色光谱发生特征性变化，这表明聚脱氧腺苷酸中形成了一种聚腺苷酸中无法形成的特定光产物。聚脱氧腺苷酸中的量子产率（φ = 2.5×10⁻³）比聚腺苷酸中的约高10倍，表明具有高结构特异性。聚脱氧腺苷酸辐照探针与聚胸苷酸（poly(dT)）的碱基配对能力受到抑制。这些结果表明DNA光损伤具有相对较高的可能性，而简单的修复酶系统无法修复这种损伤。
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