Department of Dermatology and Venereology, Peking University First Hospital, Beijing, China.
Research Center for Medical Mycology, Peking University, Beijing, China.
Methods Mol Biol. 2023;2667:159-168. doi: 10.1007/978-1-0716-3199-7_12.
Infections by dematiaceous fungi especially phaeohyphomycosis are an emerging group of infectious diseases worldwide with a variety of clinical presentations. The mouse model is a useful tool for studying phaeohyphomycosis, which can mimic dematiaceous fungal infections in humans. Our laboratory has successfully constructed a mouse model of subcutaneous phaeohyphomycosis and found significant phenotypic differences between Card9 knockout and wild-type mice, mirroring the increased susceptibility to this infection observed in CARD9-deficient humans. Here we describe construction of the mouse model of subcutaneous phaeohyphomycosis and related experiments. We hope that this chapter can be beneficial for the study of phaeohyphomycosis and facilitate the development of new diagnostic and therapeutic approaches.
暗色真菌（尤其是外瓶霉病）感染是全球范围内新出现的一组传染病，具有多种临床表现。小鼠模型是研究外瓶霉病的有用工具，可模拟人类的暗色真菌感染。我们实验室成功构建了皮下外瓶霉病的小鼠模型，并发现 Card9 敲除和野生型小鼠之间存在显著的表型差异，这与 CARD9 缺陷患者对这种感染的易感性增加相吻合。本文描述了皮下外瓶霉病小鼠模型的构建和相关实验。我们希望这一章能对研究外瓶霉病有所帮助，并促进新的诊断和治疗方法的发展。
