Matsuno Motomi, Horiuchi Junjiro, Yuasa Yoshihiro, Ofusa Kyoko, Miyashita Tomoyuki, Masuda Tomoko, Saitoe Minoru
Department of Sensory and Motor Systems, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-0057, Japan.
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGMBC), 67404 Illkirch-Graffenstaden, France, and Department of Developmental Genetics, National Institute of Genetics, Shizuoka 411-8540, Japan.
J Neurosci. 2015 Apr 8;35(14):5557-65. doi: 10.1523/JNEUROSCI.3865-14.2015.
Long-term memory (LTM) formation requires de novo gene expression in neurons, and subsequent structural and functional modification of synapses. However, the importance of gene expression in glia during this process has not been well studied. In this report, we characterize a cell adhesion molecule, Klingon (Klg), which is required for LTM formation in Drosophila. We found that Klg localizes to the juncture between neurons and glia, and expression in both cell types is required for LTM. We further found that expression of a glial gene, repo, is reduced in klg mutants and knockdown lines. repo expression is required for LTM, and expression increases upon LTM induction. In addition, increasing repo expression in glia is sufficient to restore LTM in klg knockdown lines. These data indicate that neuronal activity enhances Klg-mediated neuron-glia interactions, causing an increase in glial expression of repo. Repo is a homeodomain transcription factor, suggesting that further downstream glial gene expression is also required for LTM.
长期记忆（LTM）的形成需要神经元中从头合成基因，并随后对突触进行结构和功能修饰。然而，在此过程中神经胶质细胞中基因表达的重要性尚未得到充分研究。在本报告中，我们鉴定了一种细胞粘附分子克林贡（Klg），它是果蝇中LTM形成所必需的。我们发现Klg定位于神经元和神经胶质细胞之间的交界处，并且两种细胞类型中的表达都是LTM所必需的。我们进一步发现，神经胶质细胞基因repo在klg突变体和基因敲低品系中的表达降低。repo表达是LTM所必需的，并且在LTM诱导后表达增加。此外，增加神经胶质细胞中repo的表达足以恢复klg基因敲低品系中的LTM。这些数据表明，神经元活动增强了Klg介导的神经元-神经胶质细胞相互作用，导致神经胶质细胞中repo的表达增加。Repo是一种同源结构域转录因子，这表明LTM还需要进一步的下游神经胶质细胞基因表达。
