Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Avenida Américo Vespucio, 49, 41092 Sevilla, Spain.
J Exp Bot. 2023 Aug 17;74(15):4654-4669. doi: 10.1093/jxb/erad165.
Hydrogen sulfide (H2S) is a signaling molecule that regulates essential plant processes. In this study, the role of H2S during drought was analysed, focusing on the underlying mechanism. Pretreatments with H2S before imposing drought on plants substantially improved the characteristic stressed phenotypes under drought and decreased the levels of typical biochemical stress markers such as anthocyanin, proline, and hydrogen peroxide. H2S also regulated drought-responsive genes and amino acid metabolism, and repressed drought-induced bulk autophagy and protein ubiquitination, demonstrating the protective effects of H2S pretreatment. Quantitative proteomic analysis identified 887 significantly different persulfidated proteins between control and drought stress plants. Bioinformatic analyses of the proteins more persulfidated in drought revealed that the most enriched biological processes were cellular response to oxidative stress and hydrogen peroxide catabolism. Protein degradation, abiotic stress responses, and the phenylpropanoid pathway were also highlighted, suggesting the importance of persulfidation in coping with drought-induced stress. Our findings emphasize the role of H2S as a promoter of enhanced tolerance to drought, enabling plants to respond more rapidly and efficiently. Furthermore, the main role of protein persulfidation in alleviating reactive oxygen species accumulation and balancing redox homeostasis under drought stress is highlighted.
硫化氢（H2S）是一种信号分子，调节着植物的基本生理过程。本研究分析了 H2S 在干旱胁迫下的作用，重点研究了其潜在的作用机制。在对植物施加干旱胁迫前用 H2S 进行预处理，可显著改善植物在干旱胁迫下的典型应激表型，并降低典型的生化应激标志物如花青素、脯氨酸和过氧化氢的水平。H2S 还调节了干旱响应基因和氨基酸代谢，并抑制了干旱诱导的自噬和蛋白质泛素化，表明 H2S 预处理具有保护作用。定量蛋白质组学分析鉴定出在对照和干旱胁迫植物之间有 887 个差异显著的过硫化蛋白。对干旱胁迫下过硫化蛋白的生物信息学分析表明，最富集的生物学过程是细胞对氧化应激和过氧化氢代谢的反应。蛋白质降解、非生物胁迫反应和苯丙烷途径也被突出强调，表明过硫化在应对干旱诱导的应激中具有重要作用。本研究结果强调了 H2S 作为增强植物对干旱耐受性的促进剂的作用，使植物能够更快、更有效地做出响应。此外，还强调了蛋白质过硫化在缓解干旱胁迫下活性氧积累和平衡氧化还原平衡中的主要作用。
