Li Hua, Li Min, Wei Xingliang, Zhang Xia, Xue Ruili, Zhao Yidan, Zhao Huijie
College of Life Science, Henan Agricultural University, Zhengzhou, 450002, China.
Mol Genet Genomics. 2017 Oct;292(5):1091-1110. doi: 10.1007/s00438-017-1330-4. Epub 2017 Jun 15.
Drought is an environmental factor that deeply impacts wheat yield and quality. Hydrogen sulfide (HS) is a known regulator of drought resistance in plants. To preliminarily elucidate the regulatory mechanisms of HS on drought tolerance, the effects of HS on drought-responsive genes were investigated by transcriptome analysis. As a result, a total of 7552 transcripts not only responded to drought stress but also exhibited differential expression relative to the polyethylene glycol (PEG) treatment (P) and the NaHS pretreatment with PEG treatment (SP). GO categories of 'transport' were especially enriched under the SP treatment and ion transport categories (especially 'iron ion transport') were more significantly enriched among up-regulated transcripts in SP versus P treatments (SP.vs.P). Indeed, a higher translocation of iron from root to shoot and iron availability in shoots was detected in SP compared to P. The KEGG pathway of 'ribosome biogenesis in eukaryotes', 'protein processing in endoplasmic reticulum', 'fatty acid degradation', and 'cyanoamino acid metabolism' was induced by HS under drought stress. Further, HS was involved in plant hormones signal transduction, and drought-induced transcription factors, protein kinases, and functional genes exhibited higher expression levels under SP relative to P. Additionally, several effectors or master regulatory genes of HS were identified genome-wide. Summarily, these results showed that HS alleviated drought damage probably related to transport systems, plant hormones signal transduction, protein processing pathway, fatty acids and amino acids metabolism, which provides a guide for future experimentation to analyze hydrogen sulfide-dependent drought tolerance mechanisms in wheat.
干旱是一种对小麦产量和品质有深刻影响的环境因素。硫化氢（HS）是已知的植物抗旱调节因子。为初步阐明HS对耐旱性的调节机制，通过转录组分析研究了HS对干旱响应基因的影响。结果显示，共有7552个转录本不仅对干旱胁迫有响应，而且相对于聚乙二醇（PEG）处理（P）和PEG处理前用NaHS预处理（SP）表现出差异表达。在SP处理下，“运输”的基因本体类别尤其富集，并且在SP与P处理（SP.vs.P）的上调转录本中，离子运输类别（尤其是“铁离子运输”）的富集更为显著。事实上，与P相比，在SP中检测到铁从根到地上部的转运更高，且地上部铁的有效性更高。在干旱胁迫下，HS诱导了“真核生物中的核糖体生物合成”“内质网中的蛋白质加工”“脂肪酸降解”和“氰基氨基酸代谢”的KEGG途径。此外，HS参与植物激素信号转导，并且干旱诱导的转录因子、蛋白激酶和功能基因在SP下相对于P表现出更高的表达水平。另外，在全基因组范围内鉴定了HS的几个效应子或主调控基因。总之，这些结果表明HS减轻干旱损害可能与运输系统、植物激素信号转导、蛋白质加工途径、脂肪酸和氨基酸代谢有关，这为未来分析小麦中硫化氢依赖性耐旱机制的实验提供了指导。
