Gerweck L E, Epstein L F
Radiat Res. 1986 Jun;106(3):311-20.
The rates of cell proliferation, total protein and heat shock protein turnover, and thermotolerance decay were determined in exponential-phase CHO cells. Following a mild heat treatment of 44 degrees C for 10 min, the rate of total protein turnover slightly exceeded the rate of cell proliferation. Heated cells doubled approximately every 16 h and labeled total protein turned over with a half-time of 14 h. The turnover rate of heat shock proteins (10-h half-time) somewhat exceeded the total protein turnover rate and was similar to the thermotolerance decay rate. These data indicate that the turnover of total and heat shock proteins and thermotolerance occurs as a result of both cell division-dependent and division-independent processes.
在指数生长期的中国仓鼠卵巢（CHO）细胞中测定了细胞增殖率、总蛋白和热休克蛋白的周转速率以及耐热性衰减速率。在44℃温和热处理10分钟后，总蛋白周转速率略超过细胞增殖速率。受热细胞大约每16小时加倍一次，标记的总蛋白周转半衰期为14小时。热休克蛋白的周转速率（半衰期为10小时）略超过总蛋白周转速率，且与耐热性衰减速率相似。这些数据表明，总蛋白和热休克蛋白的周转以及耐热性是细胞分裂依赖性和非依赖性过程共同作用的结果。
