Subjeck J R, Sciandra J J, Chao C F, Johnson R J
Br J Cancer Suppl. 1982 Mar;5:127-31.
Thermotolerance is of significant interest because the resistance of pre-heated cells greatly surpasses the resistance of control cells. The induction of protein synthesis following a temperature shock (12 min, 45 degrees C) which produces thermotolerance is examined using SDS polyacrylamide gel electrophoresis. It was observed that the synthetic rates of certain proteins following heat shock significantly exceeded their synthetic rates in control (non-heat shocked) cells. The most strongly induced bands were observed at 68,000, 89,000 and 110,000 daltons. The induction of these proteins was blocked by inhibitors of transcription (actinomycin D) or translation (cycloheximide). This phenomenon strongly resembles the heat shock induction of gene transcription well studied in Drosophila melanogaster. The strong induction of gene transcription well studied in Drosophila melanogaster. The strong correlation between the kinetics of the induction of these heat shock proteins and the kinetics of thermotolerance induction is discussed.
热耐受性备受关注，因为预热细胞的抗性大大超过对照细胞的抗性。使用SDS聚丙烯酰胺凝胶电泳检测在产生热耐受性的温度冲击（12分钟，45摄氏度）后蛋白质合成的诱导情况。观察到热休克后某些蛋白质的合成速率显著超过其在对照（未热休克）细胞中的合成速率。在68,000、89,000和110,000道尔顿处观察到诱导最强的条带。这些蛋白质的诱导被转录抑制剂（放线菌素D）或翻译抑制剂（环己酰亚胺）阻断。这种现象与在黑腹果蝇中深入研究的基因转录热休克诱导非常相似。讨论了在黑腹果蝇中深入研究的基因转录的强烈诱导。这些热休克蛋白诱导动力学与热耐受性诱导动力学之间的强相关性。
