School of Chemistry and Chemical Engineering/State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Shihezi University, Shihezi 832003, China.
School of Chemistry and Chemical Engineering/State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Shihezi University, Shihezi 832003, China.
Int J Biol Macromol. 2023 Jul 1;242(Pt 1):124747. doi: 10.1016/j.ijbiomac.2023.124747. Epub 2023 May 6.
Plant diseases prompted by fungi and bacteria are one of the most serious threats to global crop production and food security. The destruction of these infections posed a major challenge to plant protection by chemical control. Herein, we develop CMCS/PA/Zn nanoparticles (NPs) using carboxymethyl chitosan (CMCS), phytic acid (PA) and metal ions (Zn) via flash nanoprecipitation (FNP) strategy. Metal complexes of PA with specified antibacterial and antifungal activities are expected to hold the potential and play a significant role in antimicrobial treatment. The size and size distribution of NPs was confirmed through Dynamic and Static Light Scatterer (DSLS). In acidic-infection microenvironment, the CMCS/PA/Zn NPs can disintegrate and release Zn in situ thus stimulated the corresponding antimicrobial activity. These CMCS/PA/Zn NPs showed outstanding antibacterial efficacy (98 %) against S. aureus and E. coli bacteria in vitro, as well as an impressive antifungal efficacy of 98 % and 81 % against R. solani and B. cinerea at 50 μg/mL respectively. This study contributes a prospective idea to the development of organic-inorganic hybrid NPs as environmentally-friendly and safe agricultural antimicrobials.
真菌和细菌引起的植物病害是全球作物生产和粮食安全面临的最严重威胁之一。这些感染的破坏对化学防治植物保护构成了重大挑战。在此，我们通过闪蒸纳米沉淀（FNP）策略使用羧甲基壳聚糖（CMCS）、植酸（PA）和金属离子（Zn）开发了 CMCS/PA/Zn 纳米颗粒（NPs）。具有特定抗菌和抗真菌活性的 PA 金属配合物有望具有潜力并在抗菌治疗中发挥重要作用。通过动态和静态光散射器（DSLS）确认了 NPs 的大小和尺寸分布。在酸性感染微环境中，CMCS/PA/Zn NPs 可以原位分解并释放 Zn，从而刺激相应的抗菌活性。这些 CMCS/PA/Zn NPs 在体外对金黄色葡萄球菌和大肠杆菌具有出色的抗菌功效（98%），在 50μg/mL 时对茄病镰刀菌和灰葡萄孢菌的抗真菌功效分别为 98%和 81%。这项研究为开发有机-无机杂化 NPs 作为环保和安全的农业抗菌剂提供了一个有前景的思路。
