School of Civil Engineering, Shandong University, Jinan, Shandong, PR China; Shandong Province Research Institute of Coal Geology Planning and Exploration, Jinan, Shandong, PR China; Laboratory of Water-sediment Regulation and eco-decontamination, Jinan, Shandong, PR China.
School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong, PR China.
Sci Total Environ. 2023 Aug 10;885:163971. doi: 10.1016/j.scitotenv.2023.163971. Epub 2023 May 6.
The challenge of managing agricultural phosphorus (P) in saline regions entails both reducing leaching for environmental protection and maintaining soil available P levels for crop production, which could be achieved through functional microorganisms that can facilitate P transformation processes like P assimilation, inorganic P solubilization, and organic P mineralization. In this study, we proposed an integrated utilization of phosphorus-accumulating bacteria (PAB) and phosphorus-solubilizing bacteria (PSB) to reach the goal of alleviating P leaching while improving soil available P levels. The study conducted a microcosm experiment that combined a soil column test, soil incubation, and pot experiment to evaluate the effect of bacterial inoculants on soil P leaching, soil P availability, and plant P accumulation. The results showed that the application of PAB reduced 22.6 % of dissolved P leaching through the absorption of labile phosphate in the soil, and 17.3 % of particulate P leaching through the promoted soil aggregation. The integrated inoculation of PSB and PAB synergistically improved soil available P content by 18.3 % through the mineralization of soil organic P, and remarkably boosted wheat growth and its P accumulation. Microbial community analysis revealed that the integrated microbial treatment decreased the diversity of soil bacterial community and increased the abundance of native microbial species, i.g. Lysobacter and Ramlibacter, which were positively correlated with soil available P content and alkaline phosphatase level. In conclusion, the integrated microbial strategy based on halotolerant PAB and PSB has great potential for sustainable P management in saline areas and agricultural activities.
在盐渍地区管理农业磷（P）的挑战既需要减少淋溶以保护环境，又需要维持土壤中可用于作物生产的有效 P 水平，这可以通过能够促进 P 转化过程（如 P 同化、无机 P 溶解和有机 P 矿化）的功能微生物来实现。在本研究中，我们提出了一种磷积累菌（PAB）和磷溶解菌（PSB）的综合利用方法，以达到减轻 P 淋溶同时提高土壤有效 P 水平的目的。该研究进行了一项微宇宙实验，结合土壤柱试验、土壤培养和盆栽试验，评估细菌接种剂对土壤 P 淋溶、土壤 P 有效性和植物 P 积累的影响。结果表明，PAB 的应用通过吸收土壤中易溶性磷酸盐减少了 22.6%的溶解 P 淋溶，通过促进土壤团聚体减少了 17.3%的颗粒 P 淋溶。PSB 和 PAB 的综合接种通过土壤有机 P 的矿化协同提高了 18.3%的土壤有效 P 含量，并显著促进了小麦的生长及其 P 积累。微生物群落分析表明，综合微生物处理降低了土壤细菌群落的多样性，增加了土著微生物物种（如 Lysobacter 和 Ramlibacter）的丰度，这些物种与土壤有效 P 含量和碱性磷酸酶水平呈正相关。总之，基于耐盐 PAB 和 PSB 的综合微生物策略在盐渍地区可持续 P 管理和农业活动中具有巨大潜力。
