Gerber F, Fournier M, Pariente R
Rev Mal Respir. 1986;3(1):31-4.
This paper is concerned with the induction of experimental allergic asthma by active sensitization of the airways "vis-à-vis" heterologous protein. Thus in the rodent it is possible to sensitize the airways by direct intra-tracheal injection of the allergen without producing an allergic alveolitis; the concomitant injection of an adjuvant is however indispensable. Confirmation of the sensitized state can be made by in vitro measurements of isometric contraction of tracheal smooth muscle in the presence of the allergen. This active sensitization is accompanied by histocytological changes which are detected by immunomorphological techniques: the rise in the number of plasma cells sensitized to IgE in the mediastinal modes: the appearance (or increase in number) of intra-cytoplasmic IgE in the sub-epithelial tracheal mast cells and in certain non ciliated intra-epithelial cells, probably basophils, morphologically close to the mast cells.
本文关注通过气道针对异源蛋白的主动致敏诱导实验性变应性哮喘。因此，在啮齿动物中，通过直接气管内注射变应原可使气道致敏而不产生变应性肺泡炎；然而，同时注射佐剂是必不可少的。致敏状态可通过在变应原存在下对气管平滑肌等长收缩的体外测量来确认。这种主动致敏伴随着通过免疫形态学技术检测到的组织细胞学变化：纵隔淋巴结中对IgE致敏的浆细胞数量增加；气管上皮下肥大细胞和某些非纤毛上皮内细胞（可能是嗜碱性粒细胞，形态上与肥大细胞相似）胞浆内IgE的出现（或数量增加）。
