Johansson S G O, Nopp A, van Hage M, Olofsson N, Lundahl J, Wehlin L, Söderström L, Stiller V, Oman H
Department of Medicine, Clinical Immunology and Allergy Unit, Karolinska Institute, Stockholm, Sweden.
Allergy. 2005 Sep;60(9):1192-9. doi: 10.1111/j.1398-9995.2005.00870.x.
To study the mechanisms of passive sensitization of patients receiving plasma containing IgE antibodies to a defined allergen.
When required for medical reasons, regular donor plasma with IgE antibodies to timothy grass allergen (8-205 kU(A)/l), was given. Kinetics of IgE antibodies in the recipients' serum and his/her basophil allergen threshold sensitivity, CD-sens, was monitored up to 2-3 weeks after transfusion. The IgE antibodies were quantitated by ImmunoCAP. The CD-sens in plasma recipients, determined by CD63 up-regulation, was measured by flow cytometry and compared to CD-sens of patients with allergic asthma and/or rhinitis.
There was a significant correlation (r = 0.98; P < 0.001) between amount of IgE antibody given and recipient serum peak concentration. The T(1/2) for IgE antibody in circulation was 1.13 days (95% confidence limit 0.35-1.91 days). The recipients became CD-sens positive already 3 h after transfusion. The CD-sens peak was observed after 3.4 days and the value were correlated (r = 0.68; P < 0.02) to the amount of IgE antibody transfused and were of the same magnitude as found in allergic patients. The T(1/2) of CD-sens indicated two populations of basophils; one with a CD-sens decrease T(1/2) of 4 days and one of 10 days.
Transfused IgE antibodies will sensitize mast cells and basophils to CD-sens levels similar to those of allergic patients. The recipients expressed 'slow' or 'rapid' CD-sens decline, indicating two different basophil populations. After transfusion of plasma with >10 kU(A)/l IgE antibody the recipient could have allergen reactive basophils for up to 7 weeks.
研究接受含有针对特定过敏原的IgE抗体的血浆的患者被动致敏机制。
因医疗原因需要时，给予含有针对梯牧草过敏原的IgE抗体的常规供体血浆（8 - 205 kU(A)/l）。在输血后长达2 - 3周内监测受者血清中IgE抗体的动力学及其嗜碱性粒细胞过敏原阈值敏感性（CD-sens）。通过免疫捕获法对IgE抗体进行定量。通过流式细胞术测量血浆受者中由CD63上调确定的CD-sens，并与过敏性哮喘和/或鼻炎患者的CD-sens进行比较。
给予的IgE抗体量与受者血清峰值浓度之间存在显著相关性（r = 0.98；P < 0.001）。循环中IgE抗体的半衰期（T(1/2)）为1.13天（95%置信区间0.35 - 1.91天）。受者在输血后3小时就已出现CD-sens阳性。在3.4天后观察到CD-sens峰值，其值与输注的IgE抗体量相关（r = 0.68；P < 0.02），且与在过敏患者中发现的值大小相同。CD-sens的半衰期表明存在两类嗜碱性粒细胞群体；一类CD-sens下降的半衰期为4天，另一类为10天。
输注的IgE抗体将使肥大细胞和嗜碱性粒细胞致敏至与过敏患者相似的CD-sens水平。受者表现出“缓慢”或“快速”的CD-sens下降，表明存在两种不同的嗜碱性粒细胞群体。输注IgE抗体>10 kU(A)/l的血浆后，受者的过敏原反应性嗜碱性粒细胞可持续长达7周。
