Gola Upasana, Kour Shilippreet, Kaur Tanvir, Perveen Kahkashan, Bukhari Najat A, Alsulami Jamilah A, Maithani Damini, Dasila Hemant, Singh Manali, Suyal Deep Chandra
Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, India.
Department of Genetics, Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, India.
Front Plant Sci. 2023 Apr 21;14:1174859. doi: 10.3389/fpls.2023.1174859. eCollection 2023.
Lantana weed ( L.) is among the most noxious weeds in the world. Keeping in mind its invasive behavior and great ecological tolerance, it becomes imperative to analyze the structure and function of associated microbiome. In this perspective, Illumina-based metagenome sequencing was performed to gain a better understanding of prokaryotic diversity and community structure in the rhizosphere soil of L. The organic carbon, nitrogen, phosphorus, and potassium contents in the rhizosphere soil were 0.91% (± 0.21%); 280 Kg ha (± 4.02 Kg ha), 54.5 Kg ha (± 3.12 Kg ha), and 189 Kg ha (± 6.11 Kg ha), respectively. The metagenome analysis revealed the existence of 41 bacterial and 2 archaeal phyla, with only 12 showing ≥1% abundances. Pseudomonadota was the dominant phylum with 31.3% abundance, followed by Actinomycetota (20.9%). Further, 54 different genera were identified with the highest abundance of (2.8%). The PICRUSt analysis predicted various functional traits in the soil metagenome, with general cellular functions dominating, followed by stress tolerance. Moreover, 10% of the functions were associated with nitrogen fixation, phosphate solubilization, and potassium mobilization. In conclusion, the present study revealed the existence of diverse prokaryotic communities in the rhizosphere of the L. which was primarily associated with stress response and plant growth promotion. To the best of our knowledge, this study documents for the first time the L. microbiome. Furthermore, the identified genera can be explored for agricultural needs in future.
马缨丹杂草（L.）是世界上最有害的杂草之一。鉴于其入侵行为和强大的生态耐受性，分析其相关微生物组的结构和功能变得势在必行。从这个角度出发，进行了基于Illumina的宏基因组测序，以更好地了解马缨丹根际土壤中的原核生物多样性和群落结构。根际土壤中的有机碳、氮、磷和钾含量分别为0.91%（±0.21%）；280千克/公顷（±4.02千克/公顷）、54.5千克/公顷（±3.12千克/公顷）和189千克/公顷（±6.11千克/公顷）。宏基因组分析揭示了41个细菌门和2个古细菌门的存在，其中只有12个门的丰度≥1%。假单胞菌门是优势门，丰度为31.3%，其次是放线菌门（20.9%）。此外，鉴定出了54个不同的属，丰度最高的是（2.8%）。PICRUSt分析预测了土壤宏基因组中的各种功能特征，其中一般细胞功能占主导，其次是胁迫耐受性。此外，10%的功能与固氮、解磷和钾的活化有关。总之，本研究揭示了马缨丹根际存在多样的原核生物群落，这些群落主要与胁迫响应和植物生长促进有关。据我们所知，本研究首次记录了马缨丹的微生物组。此外，鉴定出的属未来可用于农业需求的探索。
