Zhuansun Xiangxi, Sun Junna, Liu Nannan, Zhang Shengnan, Wang Huifang, Hu Zhaorong, Ma Jun, Sun Qixin, Xie Chaojie
Key Laboratory of Crop Heterosis and Utilization (Ministry of Education), China Agricultural University, Beijing, China.
Beijing Key Laboratory of Crop Genetic Improvement, Beijing, China.
Front Plant Sci. 2023 Apr 21;14:1108565. doi: 10.3389/fpls.2023.1108565. eCollection 2023.
Epidemics of leaf rust (caused by the fungal pathogen Erikss., ) raise concerns regarding sustainability of wheat production. Deployment of resistant cultivars is the most effective and economic strategy for combating this disease. Ofanto is a durum wheat cultivar that exhibits high resistance to race PHT throughout its entire growing period. In the present study, we identified a leaf rust resistance gene in Ofanto and temporarily designated it as . was mapped to a 2.5 cM genetic interval in chromosome arm 6BL between Indel markers and . During introgression of from Ofanto to common wheat it was observed that F plants of Ofanto crossed with Shi4185 exhibited leaf rust resistance whereas the F of Ofanto crossed with ND4503 was susceptible. In order to map the presumed suppressor locus, a Shi4185/ND4503//Ofanto three-way pentaploid population was generated and was mapped on chromosome arm 2AS. was mapped within a 2.6 cM genetic interval flanked by and . Fine mapping using 2,268 plants of the three-way cross narrowed the suppressor locus to a 68.2-kbp physical interval according to IWGSC RefSeq v1.1. Sequence analysis of genes in the physical interval revealed that encoding an RPP-13-like protein with an NB-ARC domain was a potential candidate for .
叶锈病（由真菌病原体Erikss.引起）的流行引发了人们对小麦生产可持续性的担忧。部署抗病品种是对抗这种疾病最有效且经济的策略。奥凡托是一个硬粒小麦品种，在其整个生长周期内对叶锈菌生理小种PHT表现出高抗性。在本研究中，我们在奥凡托中鉴定出一个叶锈病抗性基因，并暂时将其命名为 。该基因被定位到6BL染色体臂上Indel标记 和 之间2.5厘摩的遗传区间。在将奥凡托的 基因渗入普通小麦的过程中，观察到奥凡托与石4185杂交的F 植株表现出叶锈病抗性，而奥凡托与ND4503杂交的F 植株易感病。为了定位推测的抑制位点，构建了一个石4185/ND4503//奥凡托的三元五倍体群体，并将 定位到2AS染色体臂上。 被定位在由 和 界定的2.6厘摩遗传区间内。利用三元杂交的2268株植株进行精细定位，根据国际小麦基因组测序联盟参考序列v1.1，将抑制位点缩小到68.2千碱基对的物理区间。对该物理区间内基因的序列分析表明，编码具有NB-ARC结构域的RPP-13样蛋白的基因是 的潜在候选基因。
