Suppr超能文献

AtCuAOβ和RBOHD驱动的H₂O₂产生在伤口诱导的局部和系统性叶对叶及根对叶气孔关闭中的不同作用。

Distinct role of AtCuAOβ- and RBOHD-driven HO production in wound-induced local and systemic leaf-to-leaf and root-to-leaf stomatal closure.

作者信息

Fraudentali Ilaria, Pedalino Chiara, D'Incà Riccardo, Tavladoraki Paraskevi, Angelini Riccardo, Cona Alessandra

机构信息

Department of Science, University Roma Tre, Rome, Italy.

Istituto Nazionale Biostrutture e Biosistemi (INBB), Rome, Italy.

出版信息

Front Plant Sci. 2023 Apr 21;14:1154431. doi: 10.3389/fpls.2023.1154431. eCollection 2023.

Abstract

Polyamines (PAs) are ubiquitous low-molecular-weight aliphatic compounds present in all living organisms and essential for cell growth and differentiation. The developmentally regulated and stress-induced copper amine oxidases (CuAOs) oxidize PAs to aminoaldehydes producing hydrogen peroxide (HO) and ammonia. The CuAOβ (AtCuAOβ) was previously reported to be involved in stomatal closure and early root protoxylem differentiation induced by the wound-signal MeJA apoplastic HO production, suggesting a role of this enzyme in water balance, by modulating xylem-dependent water supply and stomata-dependent water loss under stress conditions. Furthermore, AtCuAOβ has been shown to mediate early differentiation of root protoxylem induced by leaf wounding, which suggests a whole-plant systemic coordination of water supply and loss through stress-induced stomatal responses and root protoxylem phenotypic plasticity. Among apoplastic ROS generators, the D isoform of the respiratory burst oxidase homolog (RBOH) has been shown to be involved in stress-mediated modulation of stomatal closure as well. In the present study, the specific role of AtCuAOβ and RBOHD in local and systemic perception of leaf and root wounding that triggers stomatal closure was investigated at both injury and distal sites exploiting and insertional mutants. Data evidenced that AtCuAOβ-driven HO production mediates both local and systemic leaf-to-leaf and root-to-leaf responses in relation to stomatal movement, mutants being completely unresponsive to leaf or root wounding. Instead, RBOHD-driven ROS production contributes only to systemic leaf-to-leaf and root-to-leaf stomatal closure, with mutants showing partial unresponsiveness in distal, but not local, responses. Overall, data herein reported allow us to hypothesize that RBOHD may act downstream of and cooperate with AtCuAOβ in inducing the oxidative burst that leads to systemic wound-triggered stomatal closure.

摘要

多胺(PAs)是普遍存在于所有生物体内的低分子量脂肪族化合物,对细胞生长和分化至关重要。发育调控和应激诱导的铜胺氧化酶(CuAOs)将多胺氧化为氨基醛,产生过氧化氢(HO)和氨。先前报道CuAOβ(AtCuAOβ)参与伤口信号茉莉酸甲酯(MeJA)诱导的气孔关闭和早期根原生木质部分化以及质外体HO的产生,这表明该酶在水分平衡中发挥作用,即在胁迫条件下通过调节依赖木质部的水分供应和依赖气孔的水分损失来实现。此外,AtCuAOβ已被证明介导叶片损伤诱导的根原生木质部早期分化,这表明通过胁迫诱导的气孔反应和根原生木质部表型可塑性实现了全株范围内水分供应和损失的系统协调。在质外体活性氧产生剂中,呼吸爆发氧化酶同源物(RBOH)的D亚型也已被证明参与胁迫介导的气孔关闭调节。在本研究中,利用T-DNA插入突变体,在损伤部位和远端部位研究了AtCuAOβ和RBOHD在触发气孔关闭的叶片和根部伤口的局部和系统感知中的具体作用。数据表明,AtCuAOβ驱动的HO产生介导了与气孔运动相关的局部和系统的叶对叶和根对叶反应,AtCuAOβ突变体对叶片或根部伤口完全无反应。相反,RBOHD驱动的活性氧产生仅有助于系统的叶对叶和根对叶气孔关闭,RBOHD突变体在远端而非局部反应中表现出部分无反应。总体而言,本文报道的数据使我们能够推测,RBOHD可能在诱导导致系统伤口触发气孔关闭的氧化爆发中作用于AtCuAOβ的下游并与其合作。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16ba/10160378/004ba1e1097f/fpls-14-1154431-g001.jpg

相似文献

2
A New Player in Jasmonate-Mediated Stomatal Closure: The Copper Amine Oxidase β.
Cells. 2021 Dec 2;10(12):3399. doi: 10.3390/cells10123399.
3
Leaf-Wounding Long-Distance Signaling Targets AtCuAOβ Leading to Root Phenotypic Plasticity.
Plants (Basel). 2020 Feb 15;9(2):249. doi: 10.3390/plants9020249.
5
The Apoplastic Copper AMINE OXIDASE1 Mediates Jasmonic Acid-Induced Protoxylem Differentiation in Arabidopsis Roots.
Plant Physiol. 2015 Jun;168(2):690-707. doi: 10.1104/pp.15.00121. Epub 2015 Apr 16.
6
The MeJA-inducible copper amine oxidase AtAO1 is expressed in xylem tissue and guard cells.
Plant Signal Behav. 2015;10(10):e1073872. doi: 10.1080/15592324.2015.1073872.

引用本文的文献

1
The tree of life of copper-containing amine oxidases.
Front Plant Sci. 2025 Apr 24;16:1544527. doi: 10.3389/fpls.2025.1544527. eCollection 2025.
2
AuNPs/GO/Pt microneedle electrochemical sensor for in situ monitoring of hydrogen peroxide in tomato stems in response to wounding stimulation.
Anal Bioanal Chem. 2025 Mar;417(6):1067-1079. doi: 10.1007/s00216-024-05728-5. Epub 2025 Jan 24.

本文引用的文献

3
A New Player in Jasmonate-Mediated Stomatal Closure: The Copper Amine Oxidase β.
Cells. 2021 Dec 2;10(12):3399. doi: 10.3390/cells10123399.
4
Developing climate-resilient crops: improving plant tolerance to stress combination.
Plant J. 2022 Jan;109(2):373-389. doi: 10.1111/tpj.15483. Epub 2021 Sep 22.
5
Role of Reactive Oxygen Species and Hormones in Plant Responses to Temperature Changes.
Int J Mol Sci. 2021 Aug 17;22(16):8843. doi: 10.3390/ijms22168843.
7
Crosstalk between abscisic acid and nitric oxide under heat stress: exploring new vantage points.
Plant Cell Rep. 2021 Aug;40(8):1429-1450. doi: 10.1007/s00299-021-02695-4. Epub 2021 Apr 28.
8
Abscisic Acid-Induced Stomatal Closure: An Important Component of Plant Defense Against Abiotic and Biotic Stress.
Front Plant Sci. 2021 Mar 4;12:615114. doi: 10.3389/fpls.2021.615114. eCollection 2021.
9
The Apoplast: A Key Player in Plant Survival.
Antioxidants (Basel). 2020 Jul 10;9(7):604. doi: 10.3390/antiox9070604.
10
Systemic signaling during abiotic stress combination in plants.
Proc Natl Acad Sci U S A. 2020 Jun 16;117(24):13810-13820. doi: 10.1073/pnas.2005077117. Epub 2020 May 29.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验