Division of Plant Sciences and Technology, College of Agriculture Food and Natural Resources and Interdisciplinary Plant Group, University of Missouri, Columbia, Missouri 65211, USA.
Department of Agricultural and Environmental Sciences, University Jaume I, Castelló de la Plana, 12071, Spain.
Plant Cell. 2022 Oct 27;34(11):4453-4471. doi: 10.1093/plcell/koac241.
Reactive oxygen species (ROS), produced by respiratory burst oxidase homologs (RBOHs) at the apoplast, play a key role in local and systemic cell-to-cell signaling, required for plant acclimation to stress. Here we reveal that the Arabidopsis thaliana leucine-rich-repeat receptor-like kinase H2O2-INDUCED CA2+ INCREASES 1 (HPCA1) acts as a central ROS receptor required for the propagation of cell-to-cell ROS signals, systemic signaling in response to different biotic and abiotic stresses, stress responses at the local and systemic tissues, and plant acclimation to stress, following a local treatment of high light (HL) stress. We further report that HPCA1 is required for systemic calcium signals, but not systemic membrane depolarization responses, and identify the calcium-permeable channel MECHANOSENSITIVE ION CHANNEL LIKE 3, CALCINEURIN B-LIKE CALCIUM SENSOR 4 (CBL4), CBL4-INTERACTING PROTEIN KINASE 26 and Sucrose-non-fermenting-1-related Protein Kinase 2.6/OPEN STOMATA 1 (OST1) as required for the propagation of cell-to-cell ROS signals. In addition, we identify serine residues S343 and S347 of RBOHD (the putative targets of OST1) as playing a key role in cell-to-cell ROS signaling in response to a local application of HL stress. Our findings reveal that HPCA1 plays a key role in mediating and coordinating systemic cell-to-cell ROS and calcium signals required for plant acclimation to stress.
活性氧（ROS）由质外体中的呼吸爆发氧化酶同源物（RBOHs）产生，在局部和系统细胞间信号传递中发挥关键作用，是植物适应胁迫的必需条件。在这里，我们揭示了拟南芥富含亮氨酸重复受体样激酶 H2O2 诱导的 CA2+ 增加 1（HPCA1）作为中央 ROS 受体发挥作用，需要其来传播细胞间 ROS 信号、对不同生物和非生物胁迫的系统信号、局部和系统组织的胁迫反应以及植物对胁迫的适应，这是在高光（HL）胁迫的局部处理之后发生的。我们进一步报告，HPCA1 是系统钙信号所必需的，但不是系统膜去极化反应所必需的，并且鉴定钙渗透性通道机械敏感离子通道样 3、钙调素 B 样钙传感器 4（CBL4）、CBL4 相互作用蛋白激酶 26 和蔗糖非发酵 1 相关蛋白激酶 2.6/开放气孔 1（OST1）作为传播细胞间 ROS 信号所必需的。此外，我们确定 RBOHD 中的丝氨酸残基 S343 和 S347（OST1 的假定靶标）在响应 HL 胁迫的局部应用中在细胞间 ROS 信号传递中发挥关键作用。我们的研究结果表明，HPCA1 在介导和协调植物适应胁迫所需的系统细胞间 ROS 和钙信号中发挥关键作用。
