Vickrey H M, Joseph R R, McCann D S
In Vitro. 1979 Feb;15(2):120-7. doi: 10.1007/BF02618108.
Past in vitro studies of liver-cell functions have been performed on nonproliferating primary cells or serially propagated hepatic monolayers of neoplastic or fetal origin. We optimized conditions for the selective culture of adult rabbit and canine liver parenchymal cells and presently have four differentiated proliferating monolayer strains. At the 30th passage level these hepatic cultures still display the specific liver parenchymal functions of albumin and fibrinogen synthesis as well as tyrosine aminotransferase activity. Optimization of the conditions for hepatocyte culture was monitored by [3H]thymidine incorporation. Albumin and fibrinogen synthesis were measured by bioradioimmunoassay and tryosine transaminase activity by a modification of Diamondstone's assay. Albumin and fibrinogen synthesis were correlated with hepatocyte growth kinetics.
过去对肝细胞功能的体外研究是在非增殖性原代细胞或肿瘤性或胎儿来源的连续传代培养的肝单层细胞上进行的。我们优化了成年兔和犬肝实质细胞选择性培养的条件，目前有四个分化的增殖单层细胞株。在第30代时，这些肝细胞培养物仍表现出白蛋白和纤维蛋白原合成以及酪氨酸转氨酶活性等特定的肝实质功能。通过[3H]胸腺嘧啶核苷掺入监测肝细胞培养条件的优化。白蛋白和纤维蛋白原合成通过生物放射免疫测定法测量，酪氨酸转氨酶活性通过对戴蒙德施通氏测定法的改进来测量。白蛋白和纤维蛋白原合成与肝细胞生长动力学相关。
