CIECIURA S J, MARCUS P I, PUCK T T
J Exp Med. 1956 Oct 1;104(4):615-28. doi: 10.1084/jem.104.4.615.
Tissue culture strains of cells from four different normal human tissues-liver, conjunctiva, kidney, and appendix-have been grown by the plating procedure previously developed for the HeLa strain of cervical carcinoma cells. This technique results in colony formation from isolated single cells, in a manner completely analogous to the plating of bacteria in semisolid nutrient media. Clonal cell strains have been isolated from each cell type. All behaved exactly alike in all properties studied except that some differences in plating efficiency were displayed in some of the growth media employed. The cells from normal human tissues resembled the HeLa S3 carcinomatous cell in the following properties:- (a) Single cells displayed a plating efficiency close to 100 per cent in an appropriate medium. (b) They all grew as an epithelial sheet on glass, the cells being closely packed and polygonal in shape. (c) They had mean generation times of 20 to 23 hours in the nutrient media employed, (d) The mitotic frequency was constant, and therefore the duration of mitosis was the same for all the strains studied, (e) The incidence of multinuclearity and giant formation was very low and similar in both types of cells. (f) Both classes of cells had the same total volume, and the same nuclear cross-sectional area. (g) Both also showed a tendency to spread more in the presence of human serum (concentration of 20 per cent or more) than in porcine serum. However, this differential morphological response was much more marked in the HeLa cell than in those from normal tissues. The only difference noted in the behavior of these two groups of cells lay in the tendency of the cells from normal tissues always to exhibit a greater cross-sectional area when spread on glass than the HeLa cell in the same medium. The frequency of occurrence of different types of multinuclearity in the HeLa cell and cells from normal tissues has been measured. The data suggest that multinuclearity depends on two factors: a necessary, predisposing state in the cell, and a random, independent event causing the appearance of an additional nucleus in such a prepared cell.
来自四种不同正常人体组织（肝脏、结膜、肾脏和阑尾）的细胞系通过先前为宫颈癌HeLa细胞系开发的平板接种程序进行培养。该技术可使分离的单个细胞形成集落，其方式与在半固体营养培养基中接种细菌完全类似。已从每种细胞类型中分离出克隆细胞系。除了在某些使用的生长培养基中显示出接种效率的一些差异外，所有细胞系在所有研究特性上的表现完全相同。正常人体组织的细胞在以下特性上与HeLa S3癌细胞相似：（a）单个细胞在合适的培养基中接种效率接近100%。（b）它们在玻璃上均呈上皮片状生长，细胞紧密排列且呈多边形。（c）在所使用的营养培养基中，它们的平均世代时间为20至23小时。（d）有丝分裂频率恒定，因此所有研究的细胞系的有丝分裂持续时间相同。（e）多核和巨细胞形成的发生率非常低，且在两种细胞类型中相似。（f）两类细胞的总体积相同，核横截面积也相同。（g）与猪血清相比，在人血清（浓度为20%或更高）存在的情况下，两类细胞均表现出更强的扩散倾向。然而，这种形态学差异反应在HeLa细胞中比在正常组织来源的细胞中更为明显。这两组细胞行为上唯一的差异在于，正常组织来源的细胞在相同培养基中铺展在玻璃上时，其横截面积总是比HeLa细胞大。已测量了HeLa细胞和正常组织来源的细胞中不同类型多核现象的发生率。数据表明，多核现象取决于两个因素：细胞中的一种必要的易感状态，以及一个随机、独立的事件，该事件导致处于这种准备状态的细胞中出现额外的细胞核。
