Department of Psychology, Santa Clara University, Santa Clara, California.
Curr Protoc. 2023 May;3(5):e769. doi: 10.1002/cpz1.769.
Behavioral neuroscience has long relied on in vivo electrophysiology to provide spatially and temporally precise answers to complex questions about the neural dynamics underlying sensory processing and action execution. Investigating the neural correlates of behavior can be challenging in freely behaving animals, especially when making inferences related to internal states that are temporally or conceptually ambiguous, such as decision-making or motivation. This necessitates careful creation of appropriate and rigorous controls and awareness of the many potential confounds when attributing neural signals to animal behavior. This article discusses fundamental considerations for the optimal design and interpretation of in vivo rodent electrophysiological recording experiments and focuses on the different optimization strategies required when investigating neural encoding of external stimuli versus free behavior. The first protocol offers suggestions specific to intracranial surgical implantation of multielectrode arrays. The second protocol delves into optimization strategies and tips useful for designing and interpreting recording experiments conducted in freely behaving rodents. © 2023 Wiley Periodicals LLC. Basic Protocol 1: Surgical implantation of the multielectrode array Basic Protocol 2: Optimizing experimental design and parameters.
行为神经科学长期以来一直依赖于活体电生理学，以提供有关感觉处理和动作执行的神经动力学的复杂问题的空间和时间精确答案。在自由行为的动物中研究行为的神经相关性可能具有挑战性，特别是在做出与时间或概念上模糊的内部状态相关的推断时，例如决策或动机。这需要仔细创建适当和严格的对照，并在将神经信号归因于动物行为时意识到许多潜在的混杂因素。本文讨论了优化活体啮齿动物电生理记录实验的设计和解释的基本考虑因素，并重点介绍了研究外部刺激与自由行为的神经编码时所需的不同优化策略。第一个方案提供了特定于颅内多电极阵列植入的建议。第二个方案深入探讨了优化策略和技巧，这些策略和技巧对于在自由行为的啮齿动物中进行的记录实验的设计和解释非常有用。© 2023 Wiley Periodicals LLC. 基本方案 1：多电极阵列的颅内植入 基本方案 2：优化实验设计和参数。
