Mahmud Md Muket, Kabir Ajran, Hossain Md Zawad, Mim Sanjida Jamal, Yeva Israt Jahan, Khatun Minara, Rahman Mohammad Saidur, Dey Madan Mohan, Nazir K H M Nazmul Hussain
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
These two authors contributed equally.
J Adv Vet Anim Res. 2023 Mar 31;10(1):113-117. doi: 10.5455/javar.2023.j659. eCollection 2023 Mar.
This study aimed to isolate in ready-to-cook poultry meat in Bangladesh.
Thirty drumstick samples were collected from super shops in Dhaka city ( = 10), Mymensingh city ( = 10), and Patuakhali town ( = 10). After sample processing, they were cultured in Blood agar media with base using a microfilter (0.42 nm). Suspected colonies were subjected to DNA extraction and PCR assay targeting genes. Then, sequencing was performed for confirmation.
Of 30 samples, 3 (10%) were positive for . Phylogenetic analysis shows that our isolate has strong similarities with one of the isolates from China.
The presence of this organism in ready-to-cook poultry meat is a significant concern for consumers as it bears zoonotic importance.
本研究旨在从孟加拉国即食禽肉中分离出[具体物质,原文未明确]。
从达卡市(n = 10)、迈门辛市(n = 10)和帕图阿卡利镇(n = 10)的超市收集了30个鸡腿样本。样本处理后,使用微滤器(0.42纳米)在含[具体物质,原文未明确]的基础的血琼脂培养基中培养。对疑似菌落进行DNA提取,并针对[具体基因,原文未明确]基因进行PCR检测。然后进行测序以确认。
30个样本中,3个(10%)[具体检测物质,原文未明确]呈阳性。系统发育分析表明,我们分离出的菌株与来自中国的其中一个分离株有很强的相似性。
这种生物体在即食禽肉中的存在对消费者来说是一个重大担忧,因为它具有人畜共患病的重要性。