Division of Microbiology, National Institute of Health Sciences, Kanagawa, Japan.
Lett Appl Microbiol. 2021 Jul;73(1):81-87. doi: 10.1111/lam.13478. Epub 2021 Apr 16.
We present estimations for the amounts of Arcobacter (A. butzleri, A. cryaerophilus and A. skirrowii) and Campylobacter (C. jejuni, C. coli and C. fetus) species in retail chicken, pork and beef meat using PCR-MPN. Arcobacter butzleri, A. cryaerophilus and C. jejuni were found in 100, 60 and 55% of chicken samples, respectively. No other Arcobacter or Campylobacter species were found in chicken. The MPNs of A. butzleri, A. cryaerophilus and C. jejuni were greater than 10 per 100 g in 50, 0 and 5% of samples, respectively. The MPN of A. butzleri was higher than that of C. jejuni in 95% of samples. In pork, A. butzleri and A. cryaerophilus were detected in 10 and 11 (50 and 55%) of 20 samples, respectively. No other Arcobacter or Campylobacter species were found in pork. Only one pork sample had more than 10 MPN per 100 g of A. cryaerophilus. For beef, only two samples tested positive for A. cryaerophilus, at 4600 and 92 MPN per 100 g. Overall, we found that the presence and MPNs of Arcobacter species are very high in chicken. In contrast, the positive ratios of Arcobacter in pork were high as chicken samples, but MPNs were lower than in chicken.
我们使用 PCR-MPN 方法对零售鸡肉、猪肉和牛肉中的弯曲菌（空肠弯曲菌、嗜冷弯曲菌和斯基氏弯曲菌）和弧菌（霍乱弧菌、副溶血弧菌和弯曲弧菌）的含量进行了估计。在鸡肉样本中，分别发现了 100%、60%和 55%的弯曲杆菌（A. butzleri、A. cryaerophilus 和 C. jejuni）。鸡肉中未发现其他弧菌或弯曲菌。A. butzleri、A. cryaerophilus 和 C. jejuni 的 MPN 在 50%、0%和 5%的样本中大于 10 每 100 g，分别。在 95%的样本中，A. butzleri 的 MPN 高于 C. jejuni。在猪肉中，A. butzleri 和 A. cryaerophilus 在 20 个样本中分别检测到 10 个和 11 个（50%和 55%）。猪肉中未发现其他弧菌或弯曲菌。只有一个猪肉样本的 A. cryaerophilus 超过 10 MPN 每 100 g。对于牛肉，只有两个样本 A. cryaerophilus 检测呈阳性，分别为 4600 和 92 MPN 每 100 g。总体而言，我们发现鸡肉中弧菌的存在和 MPN 非常高。相比之下，猪肉中弧菌的阳性比例与鸡肉相似，但 MPN 低于鸡肉。
