College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, China.
J Ethnopharmacol. 2023 Sep 15;313:116613. doi: 10.1016/j.jep.2023.116613. Epub 2023 May 6.
Probiotic fermentation is a mild and safe biological method to boost the performance of herbs. Portulaca oleracea L. (PO), with folklore records of purgative, anti-dermatological and anti-epidemic effects, has been demonstrated to possess anti-inflammatory, immunomodulatory, and antioxidant properties. However, the potential of PO for the treatment of atopic dermatitis (AD) has not been sufficiently explored.
This study aimed to evaluate the therapeutic benefits of PO and fermented Portulaca oleracea L. (FPO) and explore their intrinsic mechanisms.
By utilizing 2,4-dinitrofluorobenzene-induced AD mice as a model, the histopathology of the lesions was observed using H&E and toluidine blue staining methods; the levels of immunoglobulin E (Ig E), histamine (HIS), and thymic stromal lymphopoietin (TSLP) in serum were measured using ELISA, whereas, the expression of inflammatory cytokines in skin lesion was measured using ELISA and immunohistochemistry experiments. The expression of tumor necrosis factor-α (TNF-α), IKKα, NF-κB mRNA was measured using qPCR; and the expression of TNF-α、p-IKKα, p-IκBα, p-NF-κB was measured using western blotting.
Both 20 mg/mL PO and FPO alleviated mast cell infiltration and lesion pathology, reduced serum levels of Ig E, HIS and TSLP, down-regulated the expression of AD-related inflammatory cytokines, such as, TNF-α, interferon-γ, and interleukin-4, and increased filaggrin expression. Furthermore, they inhibited the expression of TNF-α, IKKα, and NF-κB genes and TNF-α, p-IKKα, p-NF-κB and p-IκBα proteins associated with the NF-κB signaling pathway.
PO and FPO has a positive therapeutic potential on AD, indicating that it may be employed as alternative therapies for AD.
益生菌发酵是一种温和且安全的生物方法，可以提高草药的性能。马齿苋（PO）具有通便、抗皮肤病和抗流行病的民间记录，已被证明具有抗炎、免疫调节和抗氧化特性。然而，PO 治疗特应性皮炎（AD）的潜力尚未得到充分探索。
本研究旨在评估 PO 和发酵马齿苋（FPO）的治疗益处，并探讨其内在机制。
利用 2,4-二硝基氟苯诱导的 AD 小鼠模型，采用 H&E 和甲苯胺蓝染色法观察病变的组织病理学；采用 ELISA 法检测血清中免疫球蛋白 E（IgE）、组织胺（HIS）和胸腺基质淋巴细胞生成素（TSLP）的水平，采用 ELISA 和免疫组化实验检测皮肤病变中炎症细胞因子的表达。采用 qPCR 法检测肿瘤坏死因子-α（TNF-α）、IKKα、NF-κB mRNA 的表达；采用 Western blot 法检测 TNF-α、p-IKKα、p-IκBα、p-NF-κB 的表达。
20mg/mL 的 PO 和 FPO 均能减轻肥大细胞浸润和病变病理学，降低血清 IgE、HIS 和 TSLP 水平，下调 AD 相关炎症细胞因子如 TNF-α、干扰素-γ和白细胞介素-4的表达，增加丝聚蛋白的表达。此外，它们抑制 TNF-α、IKKα 和 NF-κB 基因以及 TNF-α、p-IKKα、p-NF-κB 和 p-IκBα 蛋白的表达，与 NF-κB 信号通路有关。
PO 和 FPO 对 AD 具有积极的治疗潜力，表明它可能被用作 AD 的替代治疗方法。
