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引起芹菜叶斑病的完整基因组序列资源。

Complete Genome Sequence Resource for Causing Leaf Spot of Celery.

机构信息

Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin, 300192, China.

Institute of Vegetable Research, Tianjin Academy of Agricultural Sciences, Tianjin, 300192, China.

出版信息

Mol Plant Microbe Interact. 2023 Oct;36(10):670-673. doi: 10.1094/MPMI-04-23-0039-A. Epub 2023 Oct 18.

DOI:10.1094/MPMI-04-23-0039-A
PMID:37156750
Abstract

is an important seedborne pathogenic fungus causing severe Cercospora leaf spot of celery worldwide. Here, we first present a complete genome assembly of QCYBC from celery, based on Illumina paired-end and PacBio long-read sequencing data. The high-quality genome assembly contains 34 scaffolds with a 34.81 Mb genome size, 330 interspersed repeat genes, 114 noncoding RNAs, and 12,631 protein-coding genes. The benchmarking universal single-copy ortholog (BUSCO) analysis indicated that 98.2% of the BUSCOs were complete, whereas 0.3, 0.7, and 1.1% were duplicated, fragmented, and missing, respectively. Based on annotation, 508 carbohydrate-active enzymes, 243 cytochromes P450 enzymes, 1,639 translocators, 1,358 transmembrane proteins, and 1,146 virulence genes were identified. This genome sequence provides a valuable reference for future studies to improve understanding of the -celery pathosystem. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

摘要

是一种重要的种子携带致病性真菌,可导致芹菜在全球范围内发生严重的尾孢叶斑病。在这里,我们首次根据 Illumina 配对末端和 PacBio 长读测序数据,提供了来自芹菜的完整的 QCYBC 基因组组装。高质量的基因组组装包含 34 个支架,基因组大小为 34.81 Mb,330 个散布重复基因,114 个非编码 RNA 和 12631 个蛋白质编码基因。基准通用单拷贝同源物(BUSCO)分析表明,98.2%的 BUSCO 是完整的,而分别有 0.3%、0.7%和 1.1%的 BUSCO 是重复的、断裂的和缺失的。根据注释,鉴定出 508 种碳水化合物活性酶、243 种细胞色素 P450 酶、1639 种转运蛋白、1358 种跨膜蛋白和 1146 种毒力基因。该基因组序列为未来的研究提供了有价值的参考,有助于提高对 -celery 病理系统的理解。[公式:见正文] 版权所有 © 2023 作者。这是一份在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。

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