Hermiston Agricultural Research and Extension Station, Oregon State University, Hermiston, OR 19838.
Department of Plant Pathology, Washington State University, Pullman, WA 99164.
Mol Plant Microbe Interact. 2021 Aug;34(8):981-986. doi: 10.1094/MPMI-12-20-0337-A. Epub 2021 Sep 8.
is one of the most devastating pests of potato in the U.S. Pacific Northwest (PNW). Nematode-infected tubers develop external as well as internal defects, making the potato tubers unmarketable, and resulting in economic losses. Draft genome assemblies of three genotypes-race 1, race 2 and race 1 pathotype Roza-were generated using Illumina and PacBio Sequel RS II sequencing. The final assemblies consist of 30, 39, and 38 polished contigs for race 1, race 2 and race 1 pathotype Roza, respectively, with average N of 2.37 Mb and average assembled genome size of approximately 47.41 Mb. On average, 10,508 genes were annotated for each genome. Benchmarking universal single-copy ortholog (BUSCO) analysis indicated that 69.80% of the BUSCOs were complete whereas 68.80, 0.93, and 12.67% were single copy, duplicated, and fragmented, respectively. These highly contiguous genomes will enrich resources to study potato-nematode interactions and enhance breeding efforts to develop nematode-resistant potato varieties for the PNW.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
马铃薯金线虫是美国太平洋西北地区（PNW）马铃薯的最具破坏性害虫之一。感染线虫的块茎会出现外部和内部缺陷，使马铃薯块茎无法销售，造成经济损失。使用 Illumina 和 PacBio Sequel RS II 测序生成了三种基因型（1 号、2 号和 1 号致病型 Roza）的草图基因组组装。最终的组装分别由 30、39 和 38 个抛光连续体组成，1 号、2 号和 1 号致病型 Roza 的平均 N 分别为 2.37 Mb 和平均组装基因组大小约为 47.41 Mb。平均每个基因组注释了 10508 个基因。基准通用单拷贝直系同源物（BUSCO）分析表明，69.80%的 BUSCO 是完整的，而 68.80%、0.93%和 12.67%分别是单拷贝、重复和碎片化。这些高度连续的基因组将丰富资源，以研究马铃薯-线虫相互作用，并加强培育抗线虫马铃薯品种的努力，以适应 PNW。[公式：见正文]版权所有 © 2021 作者。这是一个在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。
