Fajarwati Indah, Solihin Dedy Duryadi, Wresdiyati Tutik, Batubara Irmanida
Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Jalan Agatis Kampus IPB Dramaga, Bogor 16680, Indonesia.
Divisions of Anatomy, Histology, and Embriology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Jalan Agatis Kampus IPB Dramaga, Bogor 16680, Indonesia.
Heliyon. 2023 Apr 20;9(5):e15533. doi: 10.1016/j.heliyon.2023.e15533. eCollection 2023 May.
Alloxan and streptozotocin are the most popular diabetogenic agents in assessing antidiabetic activity. Self-recovery, indicated by unstable hyperglycemia conditions in animals induced by those agents, becomes a significant disturbance to accurate examination. This study aimed to evaluate and reveal the self-recovery incidence in Sprague Dawley rats induced with alloxan and streptozotocin. Each dose of alloxan (120, 150, 180 mg/kg) and streptozotocin (40, 50, 60 mg/kg) was administered through intraperitoneal injection. The results showed that each dose of alloxan induced self-recovery incidence. In rats given streptozotocin, self-recovery only occurred at a dose of 40 mg/kg. The other higher doses of streptozotocin induced stable hyperglycemia. Furthermore, this study revealed two types of self-recovery, namely temporary recovery and end recovery. Temporary recovery occurred in rats given alloxan, during end recovery in alloxan and streptozotocin. The examination of insulin levels showed a significant reduction in the temporary recovery and stable diabetic rats compared to the end recovery rats. Besides, the bodyweight of rats was also affected by different incidences of self-recovery. This study recommends paying more attention to the possibility of self-recovery in obtaining animal models of diabetes, emphasizing the determination of suitable diabetogenic agents and proper doses to reduce self-recovery incidences. The finding of temporary recovery in rats receiving alloxan indicates that alloxan induced delayed diabetes in rats.
在评估抗糖尿病活性时，四氧嘧啶和链脲佐菌素是最常用的致糖尿病药物。由这些药物诱导的动物不稳定高血糖状况所表明的自我恢复，成为准确检测的一个重大干扰因素。本研究旨在评估和揭示用四氧嘧啶和链脲佐菌素诱导的斯普拉格-道利大鼠的自我恢复发生率。通过腹腔注射给予每剂量的四氧嘧啶（120、150、180mg/kg）和链脲佐菌素（40、50、60mg/kg）。结果表明，每剂量的四氧嘧啶都诱导了自我恢复发生率。在给予链脲佐菌素的大鼠中，自我恢复仅在40mg/kg的剂量下发生。其他较高剂量的链脲佐菌素诱导了稳定的高血糖。此外，本研究揭示了两种自我恢复类型，即暂时恢复和最终恢复。暂时恢复发生在给予四氧嘧啶的大鼠中，最终恢复发生在四氧嘧啶和链脲佐菌素组。胰岛素水平检测显示，与最终恢复的大鼠相比，暂时恢复和稳定糖尿病大鼠的胰岛素水平显著降低。此外，大鼠的体重也受到不同自我恢复发生率的影响。本研究建议在获得糖尿病动物模型时更多地关注自我恢复的可能性，强调确定合适的致糖尿病药物和适当剂量以降低自我恢复发生率。在接受四氧嘧啶的大鼠中发现暂时恢复表明四氧嘧啶诱导大鼠发生延迟性糖尿病。
