Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Faculty of Medicine and Health Science, Maulana Malik Ibrahim State Islamic University, Malang, Indonesia.
Open Vet J. 2024 Feb;14(2):730-737. doi: 10.5455/OVJ.2024.v14.i2.13. Epub 2024 Feb 29.
Controlling apoptosis induced by oxidative stress in pancreatic β-cells provides promising strategies for preventing and treating diabetes. leaves possess bioactive constituents with potential antioxidant and anti-diabetic properties.
This study aimed to investigate the molecular mechanisms by which extract protects pancreatic β-cells from apoptotic damage in streptozotocin (STZ)-induced diabetic rats.
Diabetes was induced in male Wistar rats by intraperitoneal injection of 45 mg/kg STZ, followed by 28 days of treatment with leaf extract and Glibenclamide as the standard drug. At the end of the study, blood samples were collected to measure glucose levels, oxidative stress markers, and inflammation. Pancreatic tissue was stained immunohistochemically to detect c-Jun N-terminal kinase (JNK) and Caspase-3 expression.
The administration of leaf extract to diabetic rats significantly reduced fasting blood glucose, malondialdehyde, and tumor necrosis factor-α levels, while concurrently enhancing the activity of superoxide dismutase. The immunohistochemical studies revealed a decrease in the expression of JNK and caspase-3 in the pancreatic islets of diabetic rats.
exhibits the potential to protect pancreatic β-cells from apoptosis by suppressing oxidative stress and inflammation.
控制胰腺β细胞中氧化应激诱导的细胞凋亡为预防和治疗糖尿病提供了有前景的策略。 叶具有潜在的抗氧化和抗糖尿病特性的生物活性成分。
本研究旨在探讨 提取物通过何种分子机制保护链脲佐菌素（STZ）诱导的糖尿病大鼠的胰腺β细胞免受细胞凋亡损伤。
雄性 Wistar 大鼠腹腔注射 45mg/kg STZ 诱导糖尿病，随后用 叶提取物和格列本脲（作为标准药物）治疗 28 天。研究结束时，采集血样以测量血糖水平、氧化应激标志物和炎症。用免疫组织化学法对胰腺组织进行染色，以检测 JNK 和 Caspase-3 的表达。
给予糖尿病大鼠 叶提取物可显著降低空腹血糖、丙二醛和肿瘤坏死因子-α水平，同时增强超氧化物歧化酶的活性。免疫组织化学研究显示，糖尿病大鼠胰岛中 JNK 和 caspase-3 的表达减少。
叶提取物具有通过抑制氧化应激和炎症来保护胰腺β细胞免于凋亡的潜力。
