Antoshchina M M, Luchnik N V
Tsitologiia. 1986 Apr;28(4):443-5.
Five large chromosome segments showing sometimes an abnormal staining were found in the genome of Chinese hamster (clone 237). Three types of abnormal staining were recorded. After one round of replication in the presence of BrdUrd these segments showed a hetero-staining, whereas after two rounds of replication the same segments showed iso-dark or iso-light staining. Pulse labeling with 3H-thymidine showed that all these segments were the late-replicating ones. The labeling proceeded according to all-or-none principle; in a given cell all five segments showed either presence or absence of the label. On the contrary, the abnormal staining was statistically distributed among these segments. These results are in disagreement with the current view that the abnormal staining is associated with asymmetrical distribution of thymine among two strands of DNA duplex. The above regularities are considered as an argument for the two-stranded model of chromosome.
在中国仓鼠（克隆237）的基因组中发现了五个大的染色体片段，这些片段有时显示出异常染色。记录了三种类型的异常染色。在BrdUrd存在的情况下进行一轮复制后，这些片段显示出异染色，而在两轮复制后，相同的片段显示出等暗或等亮染色。用³H-胸腺嘧啶进行脉冲标记表明，所有这些片段都是晚复制的。标记按照全或无原则进行；在给定的细胞中，所有五个片段要么显示有标记，要么显示无标记。相反，异常染色在这些片段中呈统计分布。这些结果与目前认为异常染色与胸腺嘧啶在DNA双链的两条链之间的不对称分布有关的观点不一致。上述规律被认为是支持染色体双链模型的一个论据。
