McMurray D N, Mintzer C L, Tetzlaff C L, Carlomagno M A
Tubercle. 1986 Mar;67(1):31-9. doi: 10.1016/0041-3879(86)90029-2.
Specific pathogen-free, Hartley guinea pigs were vaccinated with viable Bacille Calmette-Guerin (BCG) and given isocaloric diets identical in every nutrient except protein (control = 30%; low protein = 10%). A non-vaccinated group was maintained on the control diet. Five weeks later, all animals were infected with an aerosol containing virulent M. tuberculosis H37Rv. On the same day, half of the protein-deficient guinea pigs were transferred to the control diet, while the remainder were maintained on the low protein (10%) diet. Animals from each diet treatment were tuberculin tested and sacrificed 1,2,3 and 4 weeks post-challenge. Protein-deficient guinea pigs exhibited diminished tuberculin reactions and loss of BCG-induced protection against virulent challenge as measured by the number of viable M. tuberculosis recovered from the lung and spleen. Renourished animals expressed normal levels of delayed hypersensitivity within 1 week of initiating the normal diet and were protected as well as vaccinated control guinea pigs against virulent respiratory challenge.
将无特定病原体的Hartley豚鼠用活的卡介苗（BCG）进行接种，并给予除蛋白质外每种营养素都相同的等热量饮食（对照组 = 30%；低蛋白组 = 10%）。一个未接种疫苗的组维持对照饮食。五周后，所有动物都感染了含有强毒结核分枝杆菌H37Rv的气溶胶。在同一天，一半蛋白质缺乏的豚鼠转至对照饮食，而其余的则维持低蛋白（10%）饮食。对每种饮食处理的动物在攻毒后1、2、3和4周进行结核菌素试验并宰杀。蛋白质缺乏的豚鼠表现出结核菌素反应减弱，并且如从肺和脾脏中回收的活结核分枝杆菌数量所衡量的，卡介苗诱导的针对强毒攻击的保护作用丧失。重新给予营养的动物在开始正常饮食后1周内表现出正常水平的迟发型超敏反应，并且在针对强毒呼吸道攻击方面与接种疫苗的对照豚鼠一样受到保护。
