Considerations for Rearing and Maintaining in the Laboratory.

mosquitoes can transmit several human pathogens, including viruses such as o'nyong-nyong and parasites including spp. and spp., which cause malaria and filariasis, respectively. Rearing species of medical importance under laboratory conditions allows researchers to carry out experiments to better understand their genetics, physiology, and behavior. However, species vary in how easily they can be reared in the laboratory, and some species have been difficult to colonize. Once established, members of the important African complex thrive following a standard protocol and are predictable in growth and development rates. Here, we provide useful basic information and guidance to successfully maintain colonies of and other species of in a laboratory setting. We also provide an example of a 3-wk rearing schedule that produces sufficient numbers of mosquitoes while minimizing the work required during weekends. In the accompanying protocols, we detail efficient methods and techniques suitable for several species of this genus at the egg, larva, pupae, and adult stages; however, it will be necessary for researchers to adjust methods as needed based on site-specific rearing observations of their particular strains.