Laboratory of Radiation Oncology and Radiobiology, Clinical Oncology School of Fujian Medical University and Fujian Cancer Hospital, Fuzhou, 350014, China.
Fujian Medical University, Fuzhou, 350122, China.
Sci Rep. 2023 May 9;13(1):7523. doi: 10.1038/s41598-023-34539-4.
Gastric cancer (GC) is one of the major causes of cancer deaths with 5-year survival ratio of 20%. RNU12 is one of long noncoding RNAs (lncRNAs) regulating the tumor progression. However, how RNU12 affecting GC is not clear. qRT-PCR was utilized for determining the RNU12 expression in cell lines, 113 cases of paired gastric cancer (GC) and their adjacent normal gastric tissues. The biofunction alterations of RNU12 were assessed by its overexpression or knockdown in GC cells. MTT and cloning assay were assayed for the cell proliferation, the flow cytometry for the detection of cell cycle and the wound healing assay (WHA) and transwell invasion assay (TIA) for examining the migration and invasion of cells. The expressions of a set of genes related proliferation and migration were investigated with the Western Blotting (WB). RNA immunoprecipitation (RIP), biotinylated RNA pull-down and dual luciferase reporter tests were used to detect the interactions of RNU12 with miR-575/BLID. The in vivo proliferation and migration ability of RNU12 infected cells were determined in zebrafish system. This study revealed that RNU12 inhibited proliferation, invasion and metastasis by sponging of miR-575 and regulating the downstream BLID and modulated EMT of GC cells. The RNU12/miR-575/BLID axis is likely to be the prognosis biomarkers and drug targets of GC.
胃癌（GC）是癌症死亡的主要原因之一，5 年生存率为 20%。RNU12 是一种长链非编码 RNA（lncRNA），可调节肿瘤的进展。然而，RNU12 如何影响 GC 尚不清楚。qRT-PCR 用于确定细胞系、113 例配对胃癌（GC）及其相邻正常胃组织中的 RNU12 表达。通过在 GC 细胞中过表达或敲低 RNU12 来评估其生物功能改变。MTT 和克隆测定用于测定细胞增殖，流式细胞术用于检测细胞周期，划痕愈合测定（WHA）和 Transwell 侵袭测定（TIA）用于检测细胞迁移和侵袭。用 Western Blotting（WB）检测一组与增殖和迁移相关的基因的表达。RNA 免疫沉淀（RIP）、生物素化 RNA 下拉和双荧光素酶报告基因检测用于检测 RNU12 与 miR-575/BLID 的相互作用。在斑马鱼系统中测定感染细胞的体内增殖和迁移能力。本研究表明，RNU12 通过海绵 miR-575 抑制增殖、侵袭和转移，并调节 GC 细胞的 EMT。RNU12/miR-575/BLID 轴可能是 GC 的预后标志物和药物靶点。
