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长链非编码 RNA ZNFX1-AS1 通过调节 LIN28 和 CAPR1N1 促进胃癌细胞的侵袭和增殖。

Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1.

机构信息

Department of Clinical Laboratory, Peking University People's Hospital, Beijing 100044, China.

The Key Laboratory of Geriatrics, Peking University Fifth School of Clinical Medicine, Beijing 100730, China.

出版信息

World J Gastroenterol. 2022 Sep 14;28(34):4973-4992. doi: 10.3748/wjg.v28.i34.4973.

DOI:10.3748/wjg.v28.i34.4973
PMID:36160641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9494930/
Abstract

BACKGROUND

Long noncoding RNA (lncRNA) ZNFX1-AS1 (ZFAS1) is a newly discovered lncRNA, but its diagnostic value in gastric cancer is unclear.

AIM

To investigate the potential role of ZFAS1 in gastric cancer and to evaluate the clinical significance of ZFAS1 as a biomarker for gastric cancer screening.

METHODS

Quantitative real-time polymerase chain reaction (qRT-PCR) was used to screen for gastric cancer-associated lncRNAs in gastric cancer patients, gastric stromal tumor patients, gastritis or gastric ulcer patients, and healthy controls. Correlations between ZFAS1 expression and clinicopathological features were analyzed. The biological effects of ZFAS1 on the proliferation, migration, and invasion of gastric cancer cells were studied by MTT, colony formation, and transwell mi-gration assays. The potential mechanism of ZFAS1 was demonstrated using enzyme-linked immunosorbent assay and qRT-PCR. The relationship between ZFAS1 and tumorigenesis was demonstrated using tumor formation assays.

RESULTS

The plasma level of lncRNA ZFAS1 was significantly higher in preoperative patients with gastric cancer than in individuals in the other 4 groups. Increased expression of ZFAS1 was significantly associated with lymph node metastasis, advanced TNM stage, and poor prognosis. ZFAS1 regulated the proliferation, migration, and invasion of gastric cancer cells and regulated the growth of gastric cancer cells . LIN28 and CAPRIN1 were identified as key downstream mediators of ZFAS1 in gastric cancer cells.

CONCLUSION

LncRNA ZFAS1 promoted the invasion and proliferation of gastric cancer cells by modulating LIN28 and CAPRIN1 expression, suggesting that ZFAS1 can be used as a potential diagnostic and prognostic biomarker in gastric cancer.

摘要

背景

长链非编码 RNA (lncRNA) ZNFX1-AS1 (ZFAS1) 是一种新发现的 lncRNA，但它在胃癌中的诊断价值尚不清楚。

目的

探讨 ZFAS1 在胃癌中的潜在作用，并评估 ZFAS1 作为胃癌筛查生物标志物的临床意义。

方法

采用实时定量聚合酶链反应 (qRT-PCR) 筛选胃癌患者、胃间质瘤患者、胃炎或胃溃疡患者和健康对照者中与胃癌相关的 lncRNA。分析 ZFAS1 表达与临床病理特征的相关性。通过 MTT、集落形成和 Transwell 迁移实验研究 ZFAS1 对胃癌细胞增殖、迁移和侵袭的生物学影响。通过酶联免疫吸附试验和 qRT-PCR 验证 ZFAS1 的潜在机制。通过肿瘤形成实验证明 ZFAS1 与肿瘤发生的关系。

结果

术前胃癌患者血浆 lncRNA ZFAS1 水平明显高于其他 4 组。ZFAS1 表达增加与淋巴结转移、晚期 TNM 分期和预后不良显著相关。ZFAS1 调节胃癌细胞的增殖、迁移和侵袭，并调节胃癌细胞的生长。LIN28 和 CAPRIN1 被鉴定为 ZFAS1 在胃癌细胞中的关键下游介质。

结论

lncRNA ZFAS1 通过调节 LIN28 和 CAPRIN1 的表达促进胃癌细胞的侵袭和增殖，提示 ZFAS1 可作为胃癌潜在的诊断和预后生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/8ba1351f8ad4/WJG-28-4973-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/77642e8a81f0/WJG-28-4973-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/a90c382b09c7/WJG-28-4973-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/1b72da3e33ce/WJG-28-4973-g005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/7982ba7ef283/WJG-28-4973-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/f36b1f021606/WJG-28-4973-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/8ba1351f8ad4/WJG-28-4973-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/77642e8a81f0/WJG-28-4973-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/df2f48920e91/WJG-28-4973-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/46e9f57a2705/WJG-28-4973-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/a90c382b09c7/WJG-28-4973-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/1b72da3e33ce/WJG-28-4973-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/5c2d7b050ade/WJG-28-4973-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/ed5f7df970b0/WJG-28-4973-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/7982ba7ef283/WJG-28-4973-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/f36b1f021606/WJG-28-4973-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3ee/9494930/8ba1351f8ad4/WJG-28-4973-g010.jpg

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