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豌豆作为分析激素介导的茎伸长模型系统的 Mendel-200。

Mendel-200: Pea as a model system to analyze hormone-mediated stem elongation.

机构信息

I- Cultiver, Inc, Manteca, CA 95336 & Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA.

出版信息

Plant Signal Behav. 2023 Dec 31;18(1):2207845. doi: 10.1080/15592324.2023.2207845.

Abstract

In a recent Review Article on Gregor Mendel's (1822-1884) work with pea ()-plants, it was proposed that this crop species should be re-vitalized as a model organism for the study of cell- and organ growth. Here, we describe the effect of exogenous gibberellic acid (GA) on the growth of the second internode in 4-day-old light-grown pea seedlings (, large var. "Senator"). lnjection of glucose into the internode caused a growth-promoting effect similar to that of the hormone GA. Imbibition of dry pea seeds in GA, or water as control, resulted in a drastic enhancement in organ development in this tall variety. Similar results were reported for dwarf peas. These "classical" experimental protocols are suitable to study the elusive effect of gibberellins (which act in coordination with auxin) on the regulation of plant development at the biochemical and molecular levels.

摘要

在最近一篇关于 Gregor Mendel(1822-1884 年)豌豆植物研究工作的综述文章中,有人提议重新将这种作物作为研究细胞和器官生长的模式生物。在这里,我们描述了外源赤霉素(GA)对 4 天大的光培养豌豆幼苗(,大变种“参议员”)第二节间生长的影响。将葡萄糖注入节间会产生类似于激素 GA 的生长促进作用。将干豌豆种子浸泡在 GA 中或用作为对照的水,会导致这个高变种器官发育的急剧增强。矮生豌豆也有类似的结果。这些“经典”实验方案适用于研究赤霉素(与生长素协同作用)在生化和分子水平上对植物发育的难以捉摸的调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50a1/10177674/ac5bedec1811/KPSB_A_2207845_F0001_OC.jpg

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