Department of Biochemistry, University of Allahabad, Allahabad, India.
Rejuvenation Res. 2023 Aug;26(4):139-146. doi: 10.1089/rej.2023.0010. Epub 2023 Jun 12.
Acarbose (ACA), a well-studied and effective inhibitor of α-amylase and α-glucosidase, is a postprandial-acting antidiabetic medicine. The membrane of the erythrocyte is an excellent tool for analyzing different physiological and biochemical activities since it experiences a range of metabolic alterations throughout aging. It is uncertain if ACA modulates erythrocyte membrane activities in an age-dependent manner. As a result, the current study was conducted to explore the influence of ACA on age-dependent deteriorated functions of transporters/exchangers, disrupted levels of various biomarkers such as lipid hydroperoxides (LHs), protein carbonyl (PCO), sialic acid (SA), total thiol (-SH), and erythrocyte membrane osmotic fragility. In addition to a concurrent increase in Na/H exchanger activity and concentration of LH, PCO, and osmotic fragility, we also detected a considerable decrease in membrane-linked activities of Ca-ATPase (PMCA) and Na/K-ATPase (NKA), as well as concentrations of SA and -SH in old-aged rats. The aging-induced impairment of the activities of membrane-bound ATPases and the changed levels of redox biomarkers were shown to be effectively restored by ACA treatment.
阿卡波糖 (ACA) 是一种经过充分研究且有效的α-淀粉酶和α-葡萄糖苷酶抑制剂，属于一种餐后作用的抗糖尿病药物。红细胞膜是分析各种生理和生化活性的理想工具，因为它在衰老过程中经历了一系列代谢变化。目前尚不清楚 ACA 是否以年龄依赖的方式调节红细胞膜活性。因此，本研究旨在探讨 ACA 对年龄相关的转运体/交换器功能恶化、各种生物标志物（如脂质过氧化物 (LH)、蛋白羰基 (PCO)、唾液酸 (SA)、总巯基 (-SH)）水平的影响以及红细胞膜渗透脆性。除了 Na/H 交换器活性和 LH、PCO 和渗透脆性浓度的同步增加外，我们还检测到老年大鼠的膜结合 Ca-ATP 酶 (PMCA) 和 Na/K-ATP 酶 (NKA) 的膜连接活性以及 SA 和 -SH 浓度显著降低。研究表明，ACA 处理可有效恢复由衰老引起的膜结合 ATP 酶活性受损和氧化还原生物标志物水平变化。
