a Department of Biochemistry , University of Allahabad , Allahabad , India.
Arch Physiol Biochem. 2018 Feb;124(1):45-53. doi: 10.1080/13813455.2017.1359629. Epub 2017 Jul 31.
Erythrocyte membrane is a suitable model to study various metabolic and physiological functions as it undergoes variety of biochemical changes during aging. An age-dependent modulatory effect of rapamycin on erythrocyte membrane functions is completely unknown. Therefore, the present study was undertaken to investigate the effect of rapamycin on age-dependent impaired activities of transporters/exchangers, altered levels of redox biomarkers, viz. protein carbonyl (PC), lipid hydroperoxides (LHs), total thiol (-SH), sialic acid (SA) and intracellular calcium ion [Ca]i, and osmotic fragility of erythrocyte membrane. A significant reduction in membrane-bound activities of Na/K-ATPase (NKA) and Ca-ATPase (PMCA), and levels of -SH and SA was observed along with a simultaneous induction in Na/H exchanger (NHE) activity and levels of [Ca]i, PC, LH and osmotic fragility in old-aged rats. Rapamycin was found to be a promising age-delaying drug that significantly reversed the aging-induced impaired activities of membrane-bound ATPases and altered levels of redox biomarkers.
红细胞膜是研究各种代谢和生理功能的合适模型，因为它在衰老过程中会经历各种生化变化。雷帕霉素对红细胞膜功能的年龄依赖性调节作用尚完全未知。因此，本研究旨在探讨雷帕霉素对年龄相关的转运体/交换器活性受损、氧化还原生物标志物水平改变（如蛋白羰基（PC）、脂质过氧化物（LH）、总巯基（-SH）、唾液酸（SA）和细胞内钙离子[Ca]i）以及红细胞膜渗透脆性的影响。结果显示，老年大鼠的膜结合 Na/K-ATP 酶（NKA）和 Ca-ATP 酶（PMCA）活性以及 -SH 和 SA 水平显著降低，同时 Na/H 交换器（NHE）活性和[Ca]i、PC、LH 和渗透脆性水平升高。雷帕霉素是一种有前途的抗衰老药物，可显著逆转衰老引起的膜结合 ATP 酶活性受损和氧化还原生物标志物水平改变。
