Department of Biochemistry, University of Allahabad, Allahabad, India.
Rejuvenation Res. 2022 Jun;25(3):122-128. doi: 10.1089/rej.2021.0060.
3-Bromopyruvate (3-BP) is a glycolytic inhibitor and a potential calorie restriction mimic that shows a variety of beneficial effects in several aging model systems. A chronic low dose of 3-BP was given to male Wistar rats for 4 weeks. The effect of 3-BP on age-dependent alteration on the activities of various transporters/exchangers and redox biomarkers (protein carbonyl [PC], sialic acid [SA], sulfhydryl group [-SH], intracellular calcium ion [Ca]i, and osmotic fragility) was studied. In aged rats, 3-BP treatment increases the membrane-bound activities of Na/K-ATPase (NKA) and Ca-ATPase (PMCA), along with levels of -SH and SA. It also exerts a concomitant decrease in Na/H exchanger (NHE) activity and the levels of [Ca]i, PC, and osmotic fragility in aged groups. 3-BP can be considered as a potential antiaging agent that induces a hormetic effect leading to amelioration of age-dependent impairment of membrane-bound ATPases and alterations in the redox biomarker level.
3-溴丙酮酸（3-BP）是一种糖酵解抑制剂和潜在的热量限制模拟物，在几种衰老模型系统中显示出多种有益的效果。将慢性低剂量的 3-BP 给予雄性 Wistar 大鼠 4 周。研究了 3-BP 对各种转运体/交换体和氧化还原生物标志物（蛋白羰基[PC]、唾液酸[SA]、巯基基团[-SH]、细胞内钙离子[Ca]i 和渗透脆性）随年龄变化的影响。在老年大鼠中，3-BP 处理增加了 Na/K-ATP 酶（NKA）和 Ca-ATP 酶（PMCA）的膜结合活性，以及 -SH 和 SA 的水平。它还伴随着老年组中 NHE 活性和 [Ca]i、PC 和渗透脆性的水平降低。3-BP 可被视为一种潜在的抗衰老剂，它诱导一种有益的效应，导致膜结合 ATP 酶随年龄增长的损伤和氧化还原生物标志物水平的改变得到改善。
