Hormetic Effect of 3-Bromopyruvate on Age-Induced Alterations in Erythrocyte Membrane Transporters and Oxidative Biomarkers in Rats.

3-Bromopyruvate (3-BP) is a glycolytic inhibitor and a potential calorie restriction mimic that shows a variety of beneficial effects in several aging model systems. A chronic low dose of 3-BP was given to male Wistar rats for 4 weeks. The effect of 3-BP on age-dependent alteration on the activities of various transporters/exchangers and redox biomarkers (protein carbonyl [PC], sialic acid [SA], sulfhydryl group [-SH], intracellular calcium ion [Ca]i, and osmotic fragility) was studied. In aged rats, 3-BP treatment increases the membrane-bound activities of Na/K-ATPase (NKA) and Ca-ATPase (PMCA), along with levels of -SH and SA. It also exerts a concomitant decrease in Na/H exchanger (NHE) activity and the levels of [Ca]i, PC, and osmotic fragility in aged groups. 3-BP can be considered as a potential antiaging agent that induces a hormetic effect leading to amelioration of age-dependent impairment of membrane-bound ATPases and alterations in the redox biomarker level.