State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.
School of Ecology and Environmental Science, Yunnan University, Kunming, China.
Microb Ecol. 2023 Oct;86(3):2192-2201. doi: 10.1007/s00248-023-02237-3. Epub 2023 May 11.
To understand the disease-mediated invasion of exotic plants and the potential risk of disease transmission in local ecosystems, it is necessary to characterize population genetic structure and spatio-temporal dynamics of fungal community associated with both invasive and co-occurring plants. In this study, multiple genes were used to characterize the genetic diversity of 165 strains of Colletotrichum gloeosporioides species complex (CGSC) isolated from healthy leaves and symptomatic leaves of invasive plant Ageratina adenophora, as well as symptomatic leaves of its neighbor plants from eleven geographic sites in China. The data showed that these CGSC strains had a high genetic diversity in each geographic site (all Hd > 0.67 and Pi > 0.01). Haplotype diversity and nucleotide diversity varied greatly in individual gene locus: gs had the highest haplotype diversity (Hd = 0.8972), gapdh had the highest nucleotide diversity (Pi = 0.0705), and ITS had the lowest nucleotide diversity (Pi = 0.0074). Haplotypes were not clustered by geographic site, invasive age, or isolation source. AMOVA revealed that the genetic variation was mainly from within-populations, regardless of geographic or isolation origin. Both AMOVA and neutrality tests indicated these CGSC strains occurred gene exchange among geographic populations but did not experience population expansion along with A. adenophora invasion progress. Our data indicated that A. adenophora primarily accumulated these CGSC fungi in the introduced range, suggesting a high frequency of CGSC transmission between A. adenophora and co-occurring neighbor plants. This study is valuable for understanding the disease-mediated plant invasion and the potential risk of disease transmission driven by exotic plants in local ecosystems.
为了了解外来植物介导的疾病入侵以及疾病在当地生态系统中传播的潜在风险，有必要对与入侵植物和共生植物相关的真菌群落的种群遗传结构和时空动态进行特征描述。在这项研究中，使用多个基因来描述从入侵植物紫茎泽兰健康叶片和病叶以及来自中国 11 个地理点的 11 种伴生植物的病叶中分离出的 165 株炭疽菌复合种（CGSC）菌株的遗传多样性。数据表明，这些 CGSC 菌株在每个地理点都具有很高的遗传多样性（所有 Hd＞0.67 和 Pi＞0.01）。单倍型多样性和核苷酸多样性在单个基因座上差异很大：gs 的单倍型多样性最高（Hd＝0.8972），gapdh 的核苷酸多样性最高（Pi＝0.0705），ITS 的核苷酸多样性最低（Pi＝0.0074）。单倍型不是按地理地点、入侵年龄或分离来源聚类的。AMOVA 表明遗传变异主要来自于种群内部，无论地理或分离来源如何。AMOVA 和中性检验都表明，这些 CGSC 菌株在地理种群之间发生了基因交换，但没有随着 A. adenophora 的入侵而发生种群扩张。我们的数据表明，A. adenophora 主要在引入范围内积累了这些 CGSC 真菌，这表明 CGSC 在 A. adenophora 和伴生邻居植物之间的传播频率很高。这项研究对于了解疾病介导的植物入侵以及外来植物在当地生态系统中传播疾病的潜在风险具有重要意义。
