Bioprocess Engineering and Biotechnology Department, Federal University of Paraná Curitiba, Curitiba, 81531-980, PR, Brazil.
Federal Institute of Education, Science and Technology of Paraná (IFPR), Londrina, PR, Brazil.
World J Microbiol Biotechnol. 2023 May 11;39(7):192. doi: 10.1007/s11274-023-03641-0.
Biological contamination is one of the main bottlenecks in microalgae production, reducing quality and productivity and sometimes leading to the complete loss of the cultures. Selecting terpenes can be a pathway toward eco-friendly contamination control in microalgae cultures. This work evaluated the presence of bacterial contaminants in N. oleoabundans cultures through HTS and 16 S analysis and their susceptibility to six natural terpenes (α-pinene, β-pinene, limonene, trans-cinnamaldehyde, linalool, and eugenol). The principal phyla identified were Proteobacteria, Bacteroidetes, and Actinobacteria, and based on these data, 89 bacterial isolates of seven genera were obtained (36 Aureimonas sp., 27 Microbacterium sp., 5 Pseudomonas sp., 9 Bacillus sp., 14 Shinella sp., 1 Brevundimonas sp., and 1 Exiguobacterium sp.) at 25ºC in the presence of light. It was possible to observe that Beta-pinene 50 mg L only inhibited Bacillus sp. In contrast, Alpha-pinene, Linalool, and Trans-cinnamaldehyde, at a concentration of 6.25 mg L efficiently inhibited most isolates. The inhibition percentages found were 79-99%.
生物污染是微藻生产的主要瓶颈之一，会降低产品质量和产量，有时甚至会导致培养物完全损失。萜类化合物的选择可能是微藻培养中生态友好型污染控制的一种途径。本研究通过高通量测序（HTS）和 16S 分析评估了 N. oleoabundans 培养物中细菌污染物的存在情况，并评估了它们对六种天然萜类化合物（α-蒎烯、β-蒎烯、柠檬烯、反式肉桂醛、芳樟醇和丁香酚）的敏感性。鉴定的主要门类是变形菌门、拟杆菌门和放线菌门，根据这些数据，在 25℃光照条件下获得了来自 7 个属的 89 个细菌分离株（36 株 Aureimonas sp.、27 株 Microbacterium sp.、5 株 Pseudomonas sp.、9 株 Bacillus sp.、14 株 Shinella sp.、1 株 Brevundimonas sp.和 1 株 Exiguobacterium sp.）。结果表明，β-蒎烯 50mg/L 仅能抑制芽孢杆菌属（Bacillus sp.）。相比之下，α-蒎烯、芳樟醇和反式肉桂醛在 6.25mg/L 的浓度下能有效抑制大多数分离株，抑制率达到 79-99%。
