Feldt-Rasmussen U, Høier-Madsen M, Hansen H S, Blichert-Toft M
Acta Pathol Microbiol Immunol Scand C. 1986 Feb;94(1):33-8.
The aim of the study was to test the influence of thyroglobulin in vitro and in vivo on two different principles for quantification of serum antithyroglobulin antibody content. The methods employed were a previously described homogeneous phase radioassay and a newly developed enzyme-linked immunosorbent assay. Thyroglobulin inhibited the measurement of antithyroglobulin antibody content by both methods, not only by addition in vitro but also when present in vivo as a result of release from thyroid surgery. Both methods proved sufficiently sensitive as screening for presence of antithyroglobulin antibodies before measurement of thyroglobulin in serum. The study shows the importance of assessing the assay systems in each laboratory for interference before use, especially if intended for screening before quantifying serum thyroglobulin or for measurement of antithyroglobulin antibodies in other media (e.g. supernatants from lymphocyte cultures).
该研究的目的是在体外和体内测试甲状腺球蛋白对血清抗甲状腺球蛋白抗体含量两种不同定量原则的影响。所采用的方法是先前描述的均相放射测定法和新开发的酶联免疫吸附测定法。甲状腺球蛋白抑制了两种方法对抗甲状腺球蛋白抗体含量的测定，不仅在体外添加时如此，而且在因甲状腺手术释放而存在于体内时也是如此。在测定血清甲状腺球蛋白之前，这两种方法作为抗甲状腺球蛋白抗体存在情况的筛查方法都已证明具有足够的敏感性。该研究表明，每个实验室在使用前评估检测系统是否存在干扰非常重要，特别是如果打算在定量血清甲状腺球蛋白之前进行筛查或用于测量其他介质（如淋巴细胞培养上清液）中的抗甲状腺球蛋白抗体时。
