Gao Huayi, Liu Kun, Zhang Lu, Wang Yongfang, Fu Xubin, Guo Yujie, Bai Mei, Shen Yanbing, Wang Min
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Tianjin Ringpu Bio-technology Co., Ltd., Tianjin, China.
Front Vet Sci. 2023 Apr 24;10:1174770. doi: 10.3389/fvets.2023.1174770. eCollection 2023.
Recent studies have demonstrated the effectiveness of Gonadotropin-releasing hormone (GnRH) in inhibiting testicular growth and development in male animals to achieve castration while improving the meat quality of various livestock species, including cattle, sheep, goats, and pigs.
In this research, a GnRH-Th vaccine was synthesized using the Fmoc solid-phase synthesis technique, and the T helper (Th) antigen was modified with palmitic acid to improve its efficacy. The vaccine was then coated with a water-in-oil-in-water adjuvant to improve stability and safety. After passing safety and stability tests, the vaccine was administered to 13-week-old boars.
The results showed that it was stable, safe, and effective for up to 15 months. Moreover, the vaccine did not negatively affect the growth rate and body weight of the pigs. The palmitic acid-modified "GnRH-Th epitope peptide immunocastration vaccine (Water-in-Oil-in-Water (W/O/W)) effectively reduced the testosterone concentration and achieved castration. The concentration of androstenone and skatole hormones significantly decreased, leading to improved meat quality in the boars. The boars were then slaughtered at 33 weeks of age, and the results showed that the meat quality of the vaccinated boars was superior to that of the non-vaccinated control group ( < 0.05).
This study demonstrated that GnRH can safely and effectively achieve immune castration in boars after coupling T cell epitopes, palmitic acid modification and W-O-W coating. Provide a better method for the further development of GnRH and the realization of animal welfare.
最近的研究表明，促性腺激素释放激素（GnRH）在抑制雄性动物睾丸生长发育以实现去势方面具有有效性，同时还能改善包括牛、羊、山羊和猪在内的各种家畜的肉质。
在本研究中，采用Fmoc固相合成技术合成了一种GnRH-Th疫苗，并用棕榈酸修饰T辅助（Th）抗原以提高其效力。然后用双乳化佐剂包被该疫苗以提高稳定性和安全性。经过安全性和稳定性测试后，将该疫苗接种给13周龄的公猪。
结果表明，该疫苗在长达15个月的时间内稳定、安全且有效。此外，该疫苗对猪的生长速度和体重没有负面影响。棕榈酸修饰的“GnRH-Th表位肽免疫去势疫苗（水包油包水（W/O/W））有效降低了睾酮浓度并实现了去势。雄烯酮和粪臭素激素的浓度显著降低，从而改善了公猪的肉质。然后在33周龄时对公猪进行屠宰，结果表明接种疫苗的公猪的肉质优于未接种疫苗的对照组（<0.05）。
本研究表明，GnRH在偶联T细胞表位、棕榈酸修饰和W-O-W包被后能够安全有效地对公猪实现免疫去势。为GnRH的进一步开发和动物福利的实现提供了一种更好的方法。
