Martin-Bertram H, Hagen U
Biochim Biophys Acta. 1979 Feb 27;561(2):312-23. doi: 10.1016/0005-2787(79)90140-0.
T1 DNA, gamma-irradiated in the phage particle or irradiated with ultraviolet light was checked for structural integrity by kinetics of melting and reannealing. gamma-Irradiated DNA differed in all thermokinetic properties by a factor of 3-4 from DNA degraded by mechanical or enzymatical treatments. Ultraviolet irradiation caused much smaller effects than gamma-irradiation. Considering the frequency of pyrimidine dimers in relation to the gamma-ray induced lesions, strong evidence can be derived, that in addition to single base damages, local denatured regions are produced by gamma-irradiation. Such regions, formed possibly by direct absorption of radiation energy in DNA, i.e. by primary ionizations, are associated with base lesions and are passed over during reannealing.
通过解链和复性动力学检查噬菌体颗粒中经γ射线照射或紫外线照射的T1 DNA的结构完整性。经γ射线照射的DNA在所有热动力学性质上与经机械或酶处理降解的DNA相差3至4倍。紫外线照射产生的影响比γ射线照射小得多。考虑到嘧啶二聚体的频率与γ射线诱导的损伤的关系，可以得出有力证据，即除了单碱基损伤外，γ射线照射还会产生局部变性区域。这些区域可能是由DNA中辐射能量的直接吸收形成的，即通过初级电离形成，与碱基损伤相关，并且在复性过程中被跳过。
